In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Aminoallyl-dUTP is used for indirect stable labeling of DNA/cDNA by polymerase chain reaction (PCR) with Taq polymerase, Nick Translation with DNAse I/ DNA Polymerase I, Primer Extension with Klenow exo-, 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) and Reverse Transcription with MMLV Reverse Transcriptase. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that introduces a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks or introduces fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging and dot blots (cDNA probes) .
Aminoallyl-dUTP can also be introduced to probe by PCR and functions as a reactive site for subsequent labeling of the probe. The fluorescent labeling probe is used for in situ hybridization .
 Dirsch et al. (2007) Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes. Appl. Immunohistochem. Mol. Morphol. 3:332.
 Cox et al. (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. BioTechniques 36:114.
|Storage||Store at -20°C|
|Synonyms||5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate,aa-dutp ,aa dutp,5-AA-dUTP,5-Aminoallyl-2'-deoxyuridine-5'-Triphosphate|
|Solubility||Soluble in sterile water|
|Chemical Name||((2R,3S,5R)-5-(5-((E)-3-aminoprop-1-en-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate|
|Canonical SMILES||O=C(N1)N([[email protected]@H]2O[[email protected]](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)[[email protected]@H](O)C2)C=C(/C=C/CN)C1=O|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|