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Cy3-UTP

Catalog No.
B8330
used as a substrate for T7 RNA polymerase and generate labeled probes through in vitro transcription
Grouped product items
SizePriceStock Qty
25ul (10mM)
$240.00
Please inquire
50ul (5mM)
$240.00
Please inquire
5x25ul (10mM)
$850.00
Please inquire

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Cy3-UTP (Cyanine 3-UTP) can replace UTP as a substrate for T7 RNA polymerase and generate labeled probes through in vitro transcription. The resulting assembled mRNA will emit orange fluorescence with the involvement of Cy3-UTPs. In addition, Cy3-labeled mRNA is readily visible under ultraviolet light without further staining after gel electrophoresis[1].

Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .

Reference:

[1]Kim, H., Park, Y., & Lee, J. B. (2015). Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression. Scientific Reports, 5(1). doi:10.1038/srep12737

Chemical Properties

Physical AppearanceSolution
StorageStore at -20°C or below
M.Wt1151.98 (free acid)
FormulaC43H56N5O22P3S2(free acid)
SynonymsAminoallyl-UTP - Cy3;5-(3-Aminoallyl)-uridine-5'-triphosphate; labeled with Cy3;Triethylammonium salt;Cyanine 3-AA-UTP;Cyanine 3-Aminoallyluridine-5'-Triphosphate;Cyanine 3-UTP
SolubilitySoluble in H2O
Chemical Name2-((1E,3E)-3-(1-(6-(((E)-3-(1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(((hydroxy((hydroxy(phosphonooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)allyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylid
SDFDownload SDF
Canonical SMILESO=S(C1=CC=C([N+](CC)=C(C2(C)C)/C=C/C=C(C(C)(C)C3=C4C=CC(S(O)(=O)=O)=C3)/N4CCCCCC(NC/C=C/C5=CN([[email protected]]6[[email protected]](O)[[email protected]](O)[[email protected]@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O6)C(NC5=O)=O)=O)C2=C1)([O-])=O
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

Quality Control

Quality Control & MSDS

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Chemical structure

Cy3-UTP