EZ Cap™ mOrange mRNA (m1Ψ)

mOrange is a monomer fluorescent protein, that belongs to a variant of the DsRed family of fluorescent proteins. It is named for bright orange fluorescence and has a maximum excitation/emission spectrum of about 548/562 nm. mOrange fluorescent protein has higher photostability and faster maturation time. In addition, mOrange's spectral properties complement those of other fluorescent proteins such as GFP and mCherry, facilitating multiple labeling and analysis.

EZ Cap^TM mOrange mRNA (m1Ψ) is provided at a concentration of ~1 mg/ml with Cap1 structure. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of N1-Methylpseudo-UTP(m1Ψ) and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.