mCherry saRNA (5mCTP)

mCherry is a derivative of the red fluorescent protein DsRed, isolated from sea anemone (Discosoma). mCherry is a monomer fluorophore with a maximum absorption peak at 587 nm and a maximum emission peak at 610 nm. mCherry has good light stability and is widely used in molecular markers and cell component localization in biology.

Self-Amplifying RNA(saRNA) usually has the advantages of high protein expression, long duration, and low dose use due to its ability to self-replicate within the cell. mCherry saRNA (5mCTP) is based on the replication mechanism of Venezuelan equine encephalitis virus (VEEV)，providing a concentration of approximately 1 mg/mL with Cap1 structure. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of 5mCTP and poly (A) tail increases the stability and lifetime of the mRNA in vitro and in vivo. Poly (A) tail also plays an important role in enhancing the efficiency of translation initiation. mCherry saRNA (5mCTP) can stably express mCherry protein for a long time, which is suitable for gene expression, cell labeling and in vivo imaging studies.