EZ Cap™ mCherry mRNA (5mCTP, ψUTP)
mCherry mRNA encodes the red fluorescent protein mCherry. mCherry is a derivative of the red fluorescent protein DsRed, which is isolated from the sea anemone (Discosoma). mCherry is a monomeric fluorophore with an absorption maximum at 587 nm and an emission maximum at 610 nm. mCherry has good photostability and is widely used for molecular markers and cell component positioning in biology.
EZ Cap™ mCherry mRNA (5mCTP, ψUTP) is provided at a concentration of ~1 mg/ml with Cap1 structure. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of 5mCTP/ψUTP and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.
| mRNA Length | 996 nucleotides | ||
| Concentration | 1.0 mg/mL | ||
| Buffer | 1 mM Sodium Citrate pH 6.4 | Storage | At or below -40°C |
| Application | Reporter Genes | Cap | Cap 1 |
- 1. Zhicheng Le, Jiang Qian, et al. "A versatile gemini amphiphile-based platform with STING-activating properties for efficient gene delivery into dendritic cells." Chemical Engineering Journal Volume 497, 1 October 2024, 154513
- 2. Roach, Arantxa, et al. "Kidney-Targeted mRNA Nanoparticles: Exploration of the mRNA Loading Capacity of a Polymeric Mesoscale Platform Employing Various Classes of Excipients." (2024). Biochemistry and Molecular Biology. 2.
- 3. Ina Guri-Lamce, Yara Alrokh, et al. "Lipid nanoparticles efficiently deliver the base editor ABE8e for COL7A1 correction in dystrophic epidermolysis bullosa fibroblasts in vitro." J Invest Dermatol. 2024 Apr 5:S0022-202X(24)00269-0. PMID: 38583743
- 4. Rachel Skelton , Arantxa Roach, et al. "Formulation of Lipid‑Free Polymeric Mesoscale Nanoparticles Encapsulating mRNA." Materials Horizons. PMID: 36163410
Quality Control & Datasheet
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Related Biological Data
Related Biological Data
Related Biological Data
It can be based on your experimental goals:
- For Tracking Transfection and Translation Efficiency: APExBIO Reporter Gene mRNAs (e.g. EGFP, Firefly Luciferase mRNA) are commonly used to track transfection efficiency and protein expression duration; evaluate gene expression and cell viability; study mRNA localization and bio-distribution via in vivo imaging; optimize transfection conditions and validate LNP delivery system.
- For Gene Editing, Functional Studies and Gene Therapy Research: APExBIO offers various functional protein mRNAs, involving tumor suppressors (e.g. p53, PTEN), cytokines (e.g. IL-12, IL-10), gene-editing tools (e.g. spCas9, Cre Recombinase), gene replacement protein (e.g. EPO), and antigens (e.g. OVA, SARS-CoV-2 Spike).
- For Sustained Protein Expression: APExBIO Self-amplifying RNA (saRNA) and Circular RNA (circRNA) are recommended for applications requiring prolonged protein expression. saRNA enables lasting and strong protein expression at lower doses, while circRNA has enhanced structural stability and extended expression duration.
- Advanced Capping Technology: Utilizes Cap 1 structure (EZ Cap™ Cap) to achieve enhanced translation efficiency and minimizing activation of the host innate immune response. The capping efficiency can reach 90–99%.
- Diverse Modification Options: Provides a range of modified nucleotides, such as m1Ψ B8049, 5-moUTP B8061 and Cy5-UTP B8333, which reduce immunogenicity, improve mRNA stability, and maximize protein expression levels.
- Stringent Quality Control: Each batch undergoes rigorous quality assessment including capping efficiency, purity, integrity, and sterility to ensure batch-to-batch consistency.
