Polyethylenimine Linear(PEI), MW40000
Linear polyethylenimine (PEI, molecular weight 40,000) is a positively charged polymer widely used as a transfection reagent in in vitro cell biology and molecular biology studies. PEI condenses negatively charged DNA molecules into positively charged complexes, thereby facilitating their binding to negatively charged proteoglycans and other surface residues on the cell membrane, leading to subsequent cellular uptake through endocytosis. PEI-mediated transfection is compatible with serum-containing media, and depending on specific experimental conditions, transfection efficiency is generally over 60% to 80%. Commonly used transfection cell lines include HEK-293, HEK293T, CHO-K1, HepG2, and HeLa cells. This reagent supports applications ranging from small-scale (96-well plates) to large-scale expression systems (up to 100 liters bioreactors) and is widely used in transient expression experiments for recombinant protein production and functional gene studies.
- 1. Jing Li, Siyu Li, et al. "H3K18 lactylation-mediated nucleotide-binding oligomerization domain-2 (NOD2) expression promotes bilirubin-induced pyroptosis of astrocytes." J Neuroinflammation.2025 Mar 12;22(1):76. PMID: 40075479
- 2. Feng-Wen Niu, Ming-Dong Liu, et al. "Mitochondrial ROS-associated integrated stress response is involved in arsenic-induced blood-testis barrier disruption and protective effect of melatonin." Environ Int. 2025 Mar:197:109346. PMID: 39999483
- 3. Jing Li, Siyu Li, et al. "H3K18 lactylation-mediated nucleotide-binding oligomerization domain-2 (NOD2) expression promotes bilirubin-induced pyroptosis of astrocytes." Research Square. 12 Sep, 2024
- 4. Roach, Arantxa, et al. "Kidney-Targeted mRNA Nanoparticles: Exploration of the mRNA Loading Capacity of a Polymeric Mesoscale Platform Employing Various Classes of Excipients." (2024). Biochemistry and Molecular Biology. 2.
- 5. Yu Huang, Lu Li, et al. "WHIM Syndrome: Report of a Novel Familial CXCR4V340fs Gain-of-function Mutation with a Milder Phenotype." Research Square. 13 Nov, 2023.
| Components | 4 mL (2.5 mg/mL) | 8 mL (2.5 mg/mL) |
| PEI-2.5 mg/mL | 4 mL | 8 mL |
| 2X Transfection Buffer | 3 mL | 6 mL |
For long-term storage, store at -20°C. For frequent use, it can be placed at 4°C to avoid repeated freeze-thaw cycles. | ||