1,2-Dioleyloxy-3-dimethylamino-propane

1,2-Dioleyloxy-3-dimethylamino-propane (CAS No.: 104162-47-2) is a synthetic cationic lipid widely utilized in the development of lipid-based delivery systems for nucleic acids and small-molecule therapeutics. As a member of the ionizable lipid class, it plays a central role in the formulation of lipid nanoparticles and liposomes, where it facilitates encapsulation, cellular uptake, and endosomal escape of cargo through electrostatic interactions with negatively charged biomolecules and cellular membranes. Mechanistically, this compound has been reported to modulate cellular membrane permeability and influence intracellular signaling pathways, potentially affecting enzyme activity and membrane-associated processes, although its primary function in research is as a delivery vehicle rather than a direct pharmacological agent. In vitro studies employing similar cationic lipids typically operate in the low micromolar to sub-millimolar concentration range, with activity dependent on formulation composition and cell type. 1,2-Dioleyloxy-3-dimethylamino-propane is extensively applied in transfection protocols, gene silencing experiments, and mRNA delivery systems, as well as in preclinical models evaluating targeted drug delivery and gene therapy strategies. Experimental dosing and formulation parameters are generally optimized based on the specific biological system, payload characteristics, and desired delivery efficiency.