ARCA EGFP mRNA (5-moUTP)
EGFP (enhanced green fluorescent protein) mRNA can express an enhanced green fluorescence when transfected into cells, it is a direct-detection reporter mRNA with fluorescence emission at 509 nm, used as control to study transfection and expression in mammalian cells by various fluorescence-based assays. Our ARCA EGFP mRNA (5-moUTP) is provided at a concentration of 1mg/ml, it is ARCA capped, polyA-tailed and incorporated with modified nucleotides. Anti-Reverse Cap Analog (ARCA) is a cap analog used during in vitro transcription for the generation of capped transcripts. ARCA obtains the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with mCAP. The capping of ARCA ensures high translation efficacy, while the addition of 5-moUTP (5-Methoxy-UTP) and poly(A) tail reduces the host cell immune response and the toxicity to the modified mRNA , thus protects viable mRNA constructs. Polyadenylation (i.e. addition of Poly(A) tail) also plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation.
| mRNA Length | 996 nucleotides | ||
| Concentration | 1 mg/mL | ||
| Buffer | 1 mM Sodium Citrate, pH 6.4 | Storage | -40°C or below |
| General tips | Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent. | ||
| Shipping Condition | ship with dry ice. | ||
- 1. Lewis O'Shaughnessy, Rahman Khosravi, James Aaron Aaron Robins. "Dual end-functionalisation of poly (beta-amino ester) gene delivery vectors using multicomponent chemistry." RSC Appl. Polym., 2026
- 2. Songbo Zhao, Rongkun Li, et al. "Targeting ECM-producing cells with CAR-T therapy alleviates fibrosis in chronic kidney disease." Cell Stem Cell. 2025 Aug 18:S1934-5909(25)00271-1
- 3. Byungji Kim, Ryan R Hosn, et al. "Optimization of storage conditions for lipid nanoparticle-formulated self-replicating RNA vaccines." J Control Release. 2023 Jan:353:241-253 PMID: 36414195
Quality Control & Datasheet
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Related Biological Data
It can be based on your experimental goals:
- For Tracking Transfection and Translation Efficiency: APExBIO Reporter Gene mRNAs (e.g. EGFP, Firefly Luciferase mRNA) are commonly used to track transfection efficiency and protein expression duration; evaluate gene expression and cell viability; study mRNA localization and bio-distribution via in vivo imaging; optimize transfection conditions and validate LNP delivery system.
- For Gene Editing, Functional Studies and Gene Therapy Research: APExBIO offers various functional protein mRNAs, involving tumor suppressors (e.g. p53, PTEN), cytokines (e.g. IL-12, IL-10), gene-editing tools (e.g. spCas9, Cre Recombinase), gene replacement protein (e.g. EPO), and antigens (e.g. OVA, SARS-CoV-2 Spike).
- For Sustained Protein Expression: APExBIO Self-amplifying RNA (saRNA) and Circular RNA (circRNA) are recommended for applications requiring prolonged protein expression. saRNA enables lasting and strong protein expression at lower doses, while circRNA has enhanced structural stability and extended expression duration.
- Advanced Capping Technology: Utilizes Cap 1 structure (EZ Cap™ Cap) to achieve enhanced translation efficiency and minimizing activation of the host innate immune response. The capping efficiency can reach 90–99%.
- Diverse Modification Options: Provides a range of modified nucleotides, such as m1Ψ B8049, 5-moUTP B8061 and Cy5-UTP B8333, which reduce immunogenicity, improve mRNA stability, and maximize protein expression levels.
- Stringent Quality Control: Each batch undergoes rigorous quality assessment including capping efficiency, purity, integrity, and sterility to ensure batch-to-batch consistency.