Live-Dead Cell Staining Kit
The Live-Dead Cell Staining Kit provides fluorescent markers to distinguish between live and dead cells in cultured cell populations. It uses two complementary dyes: Calcein-AM and Propidium Iodide (PI). Calcein-AM is a membrane-permeable non-fluorescent ester that can enter intact cells and is converted to Calcein inside the cell, emitting green fluorescence (excitation/emission: ~490/515 nm). PI is a membrane-impermeable nucleic acid dye that enters compromised cells and intercalates with cellular nuclear DNA, emitting red fluorescence (excitation/emission: ~535/617 nm). Applications include viability assays for flow cytometry, fluorescence microscopy, and drug cytotoxicity or apoptosis studies. Compared to single dye or Trypan Blue methods, the dual staining approach allows for the simultaneous visualization and counting of both live and dead cell populations.
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| 500 Tests | 1000 Tests | |
| Calcein-AM Solution (2 mM) | 50 μL | 50 μL × 2 |
| PI Solution (1.5 mM) | 150 μL | 150 μL × 2 |
Store at -20°C, protect from light. Calcein-AM is easily hydrolized by moisture. Keep it desiccate and tightly close the cap after the use. | ||