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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The phage T3 RNA Polymerase, with a molecular weight of about 99 kDa, is a DNA-dependent 5’-3’ RNA polymerase, and highly specific to the T3 phage promoter (5’-AATTAACCCTCACTAAAGGGAGA-3’). This enzyme can be used to synthesize RNA strands complementary to the template DNA downstream of the T3 promoter by catalyzing the incorporation of NTP.
T3 RNA polymerase can recognize NTP substrates containing modified nucleotides such as biotin, fluorescein, digoxin labeling, commonly used in RNA hybridization probe preparation, genomic DNA sequence analysis, RNase protection assay, antisense RNA and RNAi synthesis, in vitro translation, RNA structure and function studies.
Store the components at -20 °C.