T3 RNA Polymerase (RNase-free)
Catalog No.
K1099
DNA-dependent RNA polymerase, highly specific for T3 promoter sequence
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The phage T3 RNA Polymerase, with a molecular weight of about 99 kDa, is a DNA-dependent 5’-3’ RNA polymerase, and highly specific to the T3 phage promoter (5’-AATTAACCCTCACTAAAGGGAGA-3’). This enzyme can be used to synthesize RNA strands complementary to the template DNA downstream of the T3 promoter by catalyzing the incorporation of NTP.
T3 RNA polymerase can recognize NTP substrates containing modified nucleotides such as biotin, fluorescein, digoxin labeling, commonly used in RNA hybridization probe preparation, genomic DNA sequence analysis, RNase protection assay, antisense RNA and RNAi synthesis, in vitro translation, RNA structure and function studies.
| Components | 2000 U | 5000 U | 10000 U |
| T3 RNA Polymerase (50 U/μL) | 0.04 mL | 0.1 mL | 0.2 mL |
| 10X T3 RNA Polymerase Reaction Buffer | 0.2 mL | 0.5 mL | 1 mL |
Store the components at -20 °C. | |||