HiTemp T7 RNA Polymerase (GMP-grade)

HiTemp T7 RNA Polymerase is a genetically engineered DNA-dependent RNA polymerase, which is highly specific to T7 phage promoters and reacts at higher temperatures than wild-type phage T7 RNA polymerase. The optimum reaction temperature is 50-52℃. HiTemp T7 RNA polymerase uses double-stranded DNA containing T7 promoter sequence as template, and NTP as substrate to synthesize RNA that is complementary to reverse single-stranded DNA downstream of the promoter. Linear double-stranded DNA with flat ends or 5' protruding ends, such as linear plasmid and PCR products, can be used as substrate template for HiTemp T7 RNA polymerase.

RNA synthesized by HiTemp T7 RNA polymerase covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots. The production process strictly controls host protein residues, nucleic acid residues, etc., in line with GMP production and quality management practices.