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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
α-Amanitin, the most deleterious toxin of A. phalloides to humans, inhibits RNA polymerase II (RNAPII), causing hepatic and renal failure.
Cell lines
Mouse blastocysts
Reaction Conditions
1.1 μg/ml α-amanitin
Applications
α-Amanitin inhibited the incorporation of 3H-UMP into embryos. α-Amanitin at the concentration of 1.1 μg/ml was found to inhibit RNA polymerase activity by 32%.
Animal models
Preimplantation mouse embryos at the two-cell stage of development collected from CF1 female mice
Dosage form
0 ~ 1 μg/ml α-amanitin
48 h
Less than 1% of the embryos which had been treated with 1 μg/ml of α-amanitin developed to the morula or blastocyst stages. α-Amanitin can be used to determine which forms of RNA polymerase are active and the relative amounts of each form which are present in preimplantation mouse embryos.
Note
The technical data provided above is for reference only.
References:
1. Warner CM, Versteegh LR. In vivo and in vitro effect of alpha-amanitin on preimplantation mouse embryo RNA polymerase. Nature, 1974, 248(5450): 678-680.
