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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Antibiotic; inhibits bacterial RNA polymerase. Inhibits adhesion of HUVEC cells to vitronectin (IC50 = 0.83 mM) and subsequently reduces paxillin levels. Suppresses tumor cell-induced angiogenesis.
Cell lines
Human umbilical vein endothelial cells (HUVECs), as well as breast carcinoma (MCF-7), prostate carcinoma (PC3), ovarian carcinoma (CaOV3), and small cell lung carcinoma (OH-1) cells
Reaction Conditions
0.05 ~ 500 μM thiolutin for 72 h incubation
Applications
Thiolutin potently inhibited HUVEC proliferation at 0.1 μM. The potent antiproliferative activity of thiolutin was selective for endothelial cells in that several other tumor cell lines showed weaker inhibition of proliferation by thiolutin (i.e., at 10 μM), and a small cell lung carcinoma cell line was completely resistant. Thus, thiolutin appeared to be a selective inhibitor of endothelial cell proliferation.
Note
The technical data provided above is for reference only.
References:
1. Dai S, Jia Y, Wu SL, et al. Comprehensive characterization of heat shock protein 27 phosphorylation in human endothelial cells stimulated by the microbial dithiole thiolutin. Journal of Proteome Research, 2008, 7(10): 4384-4395.