Actinomycin D
Catalog No.
A4448
RNA polymerase inhibitor
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Actinomycin D (CAS 50-76-0) is a cyclic peptide antibiotic characterized by potent anticancer and antimicrobial properties. It exerts its biological effects primarily through intercalation into DNA double helices, hindering RNA polymerase activity and thus inhibiting transcription. This mechanism effectively blocks RNA synthesis, inducing apoptosis in actively dividing cells. Due to this property, Actinomycin D is routinely utilized as a transcriptional inhibitor in molecular biology research and as a cytotoxic agent in cancer model studies to evaluate cellular responses to DNA damage and transcriptional stress.
Reference:
[1] Barret JM, Salles B, Provot C, Hill BT. Evaluation of DNA repair inhibition by antitumor or antibiotic drugs using a chemiluminescence microplate assay. Carcinogenesis. 1997 ;18(12):2441-5.
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[3] Frey U, Frey S, Schollmeier F, Krug M. Influence of actinomycin D, a RNA synthesis inhibitor, on long-term potentiation in rat hippocampal neurons in vivo and in vitro. J Physiol. 1996;490(Pt 3):703-11.
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| Storage | Desiccate at 4°C in the dark |
| M.Wt | 1255.43 |
| Cas No. | 50-76-0 |
| Formula | C62H86N12O16 |
| Synonyms | ActD |
| Solubility | ≥62.75 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
| Chemical Name | 2-amino-4,6-dimethyl-3-oxo-1-N,9-N-bis[7,11,14-trimethyl-2,5,9,12,15-pentaoxo-3,10-di(propan-2-yl)-8-oxa-1,4,11,14-tetrazabicyclo[14.3.0]nonadecan-6-yl]phenoxazine-1,9-dicarboxamide |
| SDF | Download SDF |
| Canonical SMILES | O=C(N[C@@H]1C(N[C@H](C(N2[C@]([H])(C(N(CC(N([C@H](C(O[C@@H]1C)=O)C(C)C)C)=O)C)=O)CCC2)=O)C(C)C)=O)C3=C(C(C(C)=C4OC5=C(C(C(N[C@@H]6C(N[C@H](C(N7[C@]([H])(C(N(CC(N([C@H](C(O[C@@H]6C)=O)C(C)C)C)=O)C)=O)CCC7)=O)C(C)C)=O)=O)=CC=C5C)N=C43)=O)N |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1]: | |
|
Cell lines |
Rat adipocytes |
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Preparation method |
The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20 °C for several months. |
|
Reaction Conditions |
0, 0.1, 1 or 10 μM; 24 hrs |
|
Applications |
Actinomycin D reduced the loss of leptin mRNA accumulation over the 24-hr incubation, exhibiting maximal inhibition at the concentration of 0.1 μM. |
| Animal experiment [2]: | |
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Animal models |
Wistar rats |
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Dosage form |
6 μg/μL; intrahippocampally or intracerebroventricularly |
|
Applications |
Both intrahippocampal and intracerebroventricular injection of Actinomycin D prevented a late stage of LTP in the dentate gyrus in vivo. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Fain JN, Bahouth SW. Stimulation of leptin release by actinomycin D in rat adipocytes. Biochem Pharmacol. 1998;55(8):1309-14. [2]. Frey U, Frey S, Schollmeier F, Krug M. Influence of actinomycin D, a RNA synthesis inhibitor, on long-term potentiation in rat hippocampal neurons in vivo and in vitro. J Physiol. 1996 Feb 1;490 ( Pt 3):703-11. |
|
Quality Control & MSDS
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