In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SL-327 is a selective inhibitor of MEK1 and MEK2 with IC50 values of 0.18 and 0.22μM, respectively.
MEK1 and MEK2 (ERK) are a kinase enzyme which phosphorylate mitogen-activated protein kinase (MAPK). Extracellular signal-regulated kinase (ERK) activity is essential for the acquisition of associative learning tasks.
In adult male CD-1 mice, SL-327 inhibited Pp-ERK immunostaining in the nuclei of the cells induced by cocaine. SL-327 pretreatment inhibited c-Fos expression in nuclear and inhibited activation of ERK within all the amygdala, namely LA, BLA, BMP, Ce and MePD . In morphine-pretreated rats, SL-327 increased (58%) the expression of morphine-induced psychomotor sensitization (SW3) and fully prevented the upregulation of p-PEA-15, p-FADD, and p-Akt1 at SW3 . In adult male DBA/2J mice, SL-327 significantly reduced pERK levels by 40% in both the motor cortex and dorsal striatum . In rat model, SL-327 inhibited MAPK/ERK cascade, which prevented LTP-dependent gene induction and CREB and Elk-1 phosphorylation, resulting in rapidly decaying LTP .
. Radwanska K, Caboche J, Kaczmarek L. Extracellular signal-regulated kinases (ERKs) modulate cocaine-induced gene expression in the mouse amygdala. Eur J Neurosci, 2005, 22(4): 939-948.
. Ramos-Miguel A, Esteban S, García-Sevilla JA. The time course of unconditioned morphine-induced psychomotor sensitization mirrors the phosphorylation of FADD and MEK/ERK in rat striatum: role of PEA-15 as a FADD-ERK binding partner in striatal plasticity. Eur Neuropsychopharmacol, 2010, 20(1): 49-64.
. Groblewski PA, Franken FH, Cunningham CL. Inhibition of extracellular signal-regulated kinase (ERK) activity with SL327 does not prevent acquisition, expression, and extinction of ethanol-seeking behavior in mice. Behav Brain Res, 2011, 217(2): 399-407.
. Davis S, Vanhoutte P, Pages C, et al. The MAPK/ERK cascade targets both Elk-1 and cAMP response element-binding protein to control long-term potentiation-dependent gene expression in the dentate gyrus in vivo. J Neurosci, 2000, 20(12): 4563-4572.
- 1. White SM, Avantaggiati ML, et al. "YAP/TAZ Inhibition Induces Metabolic and Signaling Rewiring Resulting in Targetable Vulnerabilities in NF2-Deficient Tumor Cells." Dev Cell. 2019 May 6;49(3):425-443.e9. PMID:31063758
- 2. Kim JW, Ko MJ, et al. "Social support rescues acute stress-induced cognitive impairments by modulating ERK1/2 phosphorylation in adolescent mice." Sci Rep. 2018 Aug 13;8(1):12003. PMID:30104581
|Storage||Store at -20°C|
|Solubility||insoluble in H2O; insoluble in EtOH; ≥16.75 mg/mL in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|
|Kinase experiment :|
Lysates (100 μg/sample) were incubated with 10 μg of an anti-MAPKAP kinase-2 antibody on a rocking platform at 4°C. After 3 h, 50 μl of a protein A/G-agarose slurry was added, and tubes were rocked for another 1 h at 4°C. Agarose beads were pelleted by centrifugation at 1500 × g for 5 min., washed three times with lysis buffer and washed once with 20 mM Hepes, pH 7.0 buffer. Immunoprecipitations were resuspended in 75 μl of kinase assay buffer (20 mM Hepes, pH 7.0, 5 mM 2-mercaptoethanol, 10 mM MgCl2, 0.1 mg/ml bovine serum albumin, containing 2 μg of hsp27. Kinase reactions were initiated by the addition of 10 μM ATP plus 10 μCi of [γ-33P]ATP and incubated at 25°C for 30 min. Reactions were terminated by the addition of Laemmli SDS sample buffer, boiled for 5 min, electrophoresed on a 12% Tris-glycine gel, dried, and quantitated using a Molecular Dynamics phosphorimager.
|Animal experiment [2,3]:|
Adult male CD-1 mice, Morphine-pretreated rats
The solubility of this compound in DMSO is >16.8mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
50 mg/kg, diluted 1:1 in water and DMSO immediately before the injection, Intraperitoneal injection,
In adult male CD-1 mice, SL-327 (50 mg/kg, i.p.) inhibited Pp-ERK immunostaining in the nuclei of the cells induced by cocaine. SL-327 (50 mg/kg, i.p.) pretreatment inhibited c-Fos expression in nuclear and inhibited activation of ERK within all the amygdala. In morphine-pretreated rats, SL-327 (20 mg/kg, i.p.) increased (58%) the expression of morphine-induced psychomotor sensitization (SW3) and fully prevented the upregulation of p-PEA-15, p-FADD, and p-Akt1 at SW3.
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
. Scherle P A, Ma W, Lim H, et al. Regulation of Cyclooxygenase-2 Induction in the Mouse Uterus During Decidualization AN EVENT OF EARLY PREGNANCY[J]. Journal of Biological Chemistry, 2000, 275(47): 37086-37092.
. Radwanska K, Caboche J, Kaczmarek L. Extracellular signal‐regulated kinases (ERKs) modulate cocaine‐induced gene expression in the mouse amygdala[J]. European Journal of Neuroscience, 2005, 22(4): 939-948.
 Ramos-Miguel A, Esteban S, García-Sevilla J A. The time course of unconditioned morphine-induced psychomotor sensitization mirrors the phosphorylation of FADD and MEK/ERK in rat striatum: role of PEA-15 as a FADD-ERK binding partner in striatal plasticity[J]. European Neuropsychopharmacology, 2010, 20(1): 49-64.
|Description||SL-327 is a selective inhibitor of MEK1 and MEK2 with IC50 value of 0.18 and 0.22 μM, respectively.|
|IC50||0.18 μM||0.22 μM|
Quality Control & MSDS
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