In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Myriocin is a potent immunosuppressant and also a specific serine palmitoyltransferase inhibitor with Ki value of 0.28 nM .
Serine palmitoyltransferase catalyzes the first step in de novo sphingolipid biosynthesis. Sphingolipids are involved in the regulation of apoptosis, cell proliferation, cell migration, senescence and inflammation .
Myriocin is a specific serine palmitoyltransferase inhibitor. In lung cancer A549 and NCI-H460 cells, myriocin (10, 20, and 50 ?M) inhibited cell proliferation in a concentration-dependent manner. Myriocin inhibited A549 and NCI-H460 cell growth with IC50 values of 30 ?M and 26 ?M, respectively .
In melanoma mice, myriocin (1 mg/kg, every other day for 3 weeks) administered intradermally or intraperitoneally significantly inhibited tumor formation. Myriocin also reduced the expression of Cdc25C, Cdc2 and cyclin B1 in tumor tissues, and increased the expression of p53 and p21waf1/cip1. Myriocin also decreased the levels of sphingolipids in serum, liver and tumor tissue . In BALB/c mice, myriocin increased dopamine (DA) in striatum and hippocampus and reduced it in cortex .
Miyake Y, Kozutsumi Y, Nakamura S, et al. Serine palmitoyltransferase is the primary target of a sphingosine-like immunosuppressant, ISP-1/myriocin. Biochem Biophys Res Commun. 1995 Jun 15;211(2):396-403.
Osuchowski MF, Johnson VJ, He Q, et al. Myriocin, a serine palmitoyltransferase inhibitor, alters regional brain neurotransmitter levels without concurrent inhibition of the brain sphingolipid biosynthesis in mice. Toxicol Lett. 2004 Feb 28;147(1):87-94.
Lee YS, Choi KM, Lee S, et al. Myriocin, a serine palmitoyltransferase inhibitor, suppresses tumor growth in a murine melanoma model by inhibiting de novo sphingolipid synthesis. Cancer Biol Ther. 2012 Jan 15;13(2):92-100.
Choi KE, Jung YS, Kim DH, et al. Myriocin induces apoptotic lung cancer cell death via activation of DR4 pathway. Arch Pharm Res. 2014 Apr;37(4):501-11.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||2mg/mL in methanol|
|Chemical Name||(2S,3R,4R,E)-2-amino-3,4-dihydroxy-2-(hydroxymethyl)-14-oxoicos-6-enoic acid|
|Canonical SMILES||CCCCCCC(CCCCCC/C([H])=C([H])/C[[email protected]](O)([H])[[email protected]@](O)([H])[[email protected]@](C(O)=O)(N)CO)=O|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37°C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.|