BDNF, human recombinant
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Human Brain-Derived Neurotrophic Factor (BDNF) is a member of the NGF family of neurotrophic factors and supports the growth and survivability of nerve and glial cells. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5, which are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The active form of BDNF is a 27.0 kDa dimer, formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions. BDNF is highly expressed in the cerebellum, hippocampus, placenta and fetal eye. BDNF binds to and activates the TrkB tyrosine kinase receptor with high affinity and specificity.
| Determined by its ability to stimulate chick E8 neurite outgrowth. | ||||||
| ED50 | 0.1-1.0 ng/ml. | |||||
Quality Control & DataSheet
- View current batch:
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Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
Related Biological Data
| Gene ID: | 627 | Accession #: | P23560 |
| Alternate Names: | human BDNF, BDNF, h-BDNF, rh-BDNF, recombinant human BDNF, recombinant BDNF. | ||
| Source: | Escherichia Coli. | M.Wt: | 27.0 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20°C |
| Formulation: | Sterile filtered and lyophilized with no additives. | ||
| Solubility: | Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use. | ||
| Shipping Condition: | gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||