BDNF, human recombinant
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly, to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.
| Gene ID | |
| Accession # | |
| Alternate Names | |
| Source | Spodoptera frugiperda, Sf 21 (baculovirus) |
| M.Wt | Apparent molecular mass of 13-14 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 119 amino acids. |
| AA Sequence | His129-Arg247; Accession # P23560 |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from 0.2 µm filtered concentrated solution in 100 mM Sodium Citrate and 300 mM NaCl, pH 3.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1 mg/mL. Further dilutions should be made in appropriately buffered solutions. |
| Biological Activity | Testing in progress.Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL. The specific activity of rHu/Mu/Rt/Ca/EqBDNF is approximately 1.3 × 103 units/μg, which is calibrated against recombinant human BDNF WHO Standard. Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera is coated at 1 μg/mL, rHu/Mu/Rt/Ca/EqBDNF binds with an apparent Kd <1 nM. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 97 % by SDS-PAGE analyses.
- Datasheet
Endotoxin: Less than 0.1 EU/µg of rHu/Mu/Rt/Ca/EqBDNF as determined by LAL method.