DdeI

DdeI is a genetically engineered fast restriction endonuclide, which is suitable for fast enzyme digestion of plasmid DNA, PCR products or genomic DNA, etc., and can complete the reaction within 5-15 min. Its internal matching Reaction Buffer (Color) contains red and yellow tracer dyes, which can directly use the product for gel electrophoresis. The migration rate of red dye and 2500 bp double-stranded DNA fragment in 1% agarose gel was similar, and the migration rate of yellow dye and 10 bp double-stranded DNA fragment in 1% agarose gel was similar.

Its recognition sequence and cutting site are as follows:

5'……C^↓TNAG……3'
3'……GANT_↑C……5'