G-CSF, murine recombinant
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine that is mainly produced by monocytes and macrophages upon activation by TNF-alpha and IFN-gamma and endotoxin. G-CSF plays an important role in the activation, proliferation and differentiation of hematopoietic cells of the neutrophilic granulocyte lineage and the mobilization of hematopoietic stem cells and progenitors into the blood circulation.
The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein that is proteolytically cleaved to generate the 178 aa residue mature protein. Mouse G-CSF and human G-CSF show species cross-reactivity and mouse G-CSF is active on human cells. Recombinant murine G-CSF is a 19.0 kDa protein consisting of 179 amino acid residues.
| Determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells. | ||||||
| ED50 | < 10-60 ng/ml. | |||||
Quality Control & DataSheet
- View current batch:
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Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
Related Biological Data
Related Biological Data
| Gene ID: | 12985 | Accession #: | P09920 |
| Alternate Names: | CSF3, Murine G-CSF, G-CSF, G CSF, GCSF, m-G-CSF, rm-G-CSF, recombinant murine G-CSF, recombinant G-CSF. | ||
| Source: | Escherichia Coli. | M.Wt: | 19.0 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20°C |
| Formulation: | Sterile filtered and lyophilized from 5 mM NaCitrate, pH 4.0. | ||
| Solubility: | Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use. | ||
| Shipping Condition: | Gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||