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Sulfo-NHS-LC-BiotinAmine-reactive biotinylation agent

Sulfo-NHS-LC-Biotin

Catalog No. A8003
Size Price Stock Qty
100mg $80.00 In stock
1g $450.00 In stock

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Sample solution is provided at 25 µL, 10mM.

Product Citations

1.Lorin, Charlotte, Isabelle Vögeli, and Ernst Niggli. "Dystrophic cardiomyopathy: role of TRPV2 channels in stretch-induced cell damage." Cardiovascular research 106.1 (2015): 153-162. PMID:25616416

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Chemical structure

Sulfo-NHS-LC-Biotin

Related Biological Data

Sulfo-NHS-LC-Biotin

Biological Activity

Description Sulfo-NHS-LC-Biotin is an intermediate length, water-soluble biotinyltation reagent used to attach biotin to primary amines.
Targets            
IC50            

Protocol

Biotinylation method [1]:

Sample

Cardiomyocytes

Preparation method

Soluble in Water. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

0.5 mg/ml, 37℃ for 2 h,

Applications

Sulfo-NHS-LC-Biotin was dissolved in PBS at a concentration of 0.5 mg/ml. then it was incubated with cardiomyocytes at 37℃ for 2 h. After that the cells were washed twice in cold PBS with 100 mM glycine and twice in cold PBS alone to remove the excess biotin reagent. To capture biotinylated proteins, 100 μl of streptavidin agarose resin were incubated with each 100 μg of samples for 16 hours at 4℃. Captured proteins were eluted at 60℃ for 30 min with Laemmli 2X sample buffer which contain 40 mg/mL of dithiothreitol and they were analyzed by Western blot with primary antibodies.

References:

[1]. Charlotte Lorin, Isabelle Vigeli, Ernst Niggli. Dystrophic cardiomyopathy - role of TRPV2 channels in stretch-induced cell damage. Cardiovascular Research, 2015.

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Chemical Properties

Cas No. 127062-22-0 SDF Download SDF
Synonyms Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt
Chemical Name 2,5-dioxo-1-[6-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoylamino]hexanoyloxy]pyrrolidine-3-sulfonic acid;sodium
Canonical SMILES C1C(C(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)O.[Na]
Formula C20H29N4NaO9S2 M.Wt 556.6
Solubility Soluble in Water Storage Store at 4°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

Background

Sulfo-NHS-LC-biotin (Sulfosuccinimidyl 6-(biotinamido) Hexanoate) is an amine-reactive biotinylation agent. As a water-soluble analog of NHS-LC-biotin, Sulfo-NHS-LC-biotin contains a negatively charged sulfonate group on the NHS ring structure, which creates sufficient polarity within the molecule and allows it to be directly added into aqueous reactions without prior dissolution of organic solvents. Sulfo-NHS-LC-biotin covalently binds to amine groups in proteins and other molecules through its NHS ester forming an amide linkage and releasing N-Hydroxysuccinimide. Sulfo-NHS-LC-biotin has a greater length (22.4 A) between a covalently modified molecule and the bicyclic biotin rings than NHS-biotin (13.5 A), which provides the probes a greater opportunity to reach the binding sites deeply buried inside the surface plane of proteins.

Reference

Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson  (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL).  Academic Press  (an imprint of Elsevier):  London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.