Sulfo-NHS-SS-Biotin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Sulfo-NHS-SS-biotin(sulfosuccinimidyl-20(biotinamido)ethyl-1,3-dithiopropionate) is a long-chain cleavable amine-reactive biotinylation reagent. The presence of the negatively charged sulfonate group in the chemical structure of sulfo-NHS-SS-biotin makes it a water-soluble biotinylation reagent that can be directly added to aqueous reactions without prior dissolution of organic solvents. Although no prior dissolution is required, an aqueous stock solution of sulfo-NHS-SS-biotin must be prepared rapidly and used immediately in case of the occurrence of hydrolysis of the active ester. Sulfo-NHS-SS-biotin has been used to react with amine-containing proteins and other molecules forming a complex which further interacts with avidin or streptavidin probes and to purify targeted molecules using affinity chromatography on a column of immobilized avidin or streptavidin.
Reference
Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
- 1. Yang Y, Yu Q, et al. "Decreased NHE3 activity and trafficking in TGEV-infected IPEC-J2 cells via the SGLT1-mediated P38 MAPK/AKt2 pathway." Virus Res. 2020;280:197901. PMID:32070687
- 2. Saladi S, Boos F, et al. "The NADH Dehydrogenase Nde1 Executes Cell Death after Integrating Signals from Metabolism and Proteostasis on the Mitochondrial Surface." Mol Cell. 2019 Oct 18. pii: S1097-2765(19)30732-4. PMID:31668496
- 3. Sheridan J.S. Carrington. "Differential Glycosylation of the Inwardly Rectifying Potassium Channel Kir7.1 by G protein-coupled Receptors." Vanderbilt University.2019.
- 4. Carrington S, Hernandez C, et al. "G protein-coupled receptors differentially regulate glycosylation and activity of the inwardly rectifying potassium channel Kir7.1." J Biol Chem. 2018 Sep 26. pii: jbc.RA118.003238. PMID:30257863
- 5. Brasher MI, Martynowicz DM, et al. "Interaction of Munc18c and Syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumour cells." J Biol Chem. 2017 Aug 10. pii: jbc.M117.807438. PMID:28798239
• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the N-terminal-amine
• Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Medium length—spacer arm is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain
| Storage | Store at -20°CThe product is not stable in solution, please dissolve it immediately before use. |
| M.Wt | 606.7 |
| Cas No. | 325143-98-4 |
| Formula | C19H27N4NaO9S4 |
| Synonyms | Biotin disulfide N-hydroxysulfosuccinimide ester |
| Chemical Name | sodium;1-[3-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethyldisulfanyl]propanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate |
| SDF | Download SDF |
| Canonical SMILES | C1C(C(=O)N(C1=O)OC(=O)CCSSCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+] |
| Shipping Condition | Ship with blue ice, or upon other requests. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon. |
| Biotinylation method [1]: | |
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Sample |
468/EV cells and 468/uPAR cells. |
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Preparation method |
Soluble in water, DMSO or DMF. |
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Reaction Conditions |
1 mg/ml, 15minutes on ice |
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Applications |
Cells in monolayer culture (1.5 × 106) were washed three times with ice-cold PBS and then treated with sulfo-NHS-SS-biotin (1mg/mL) for 15 minutes on ice. Biotinylation reactions were terminated with 100 mmol/L glycine in PBS. After washing with PBS, cell extracts were prepared in radioimmunoprecipitation assay (RIPA) buffer (20 mmol/L sodium phosphate, 150 mmol/L NaCl (pH 7.4), 1% NP40, 0.1% SDS, and 0.5% deoxycholic acid) with protease inhibitor cocktail. Biotinylated membrane proteins were precipitated with streptavidin-sepharose. Proteins were eluted with SDS sample buffer, resolved by SDS-PAGE, electrotransferred to polyvinylidene difluoride (PVDF) membranes, and probed with primary antibodies. |
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References: [1]. Minji Jo, Boryana M. Eastman, Drue L. Webb, et al. Cell Signaling by Urokinase-type Plasminogen Activator Receptor Induces Stem Cell-like Properties in Breast Cancer cells . Cancer Res, 2010;70:8948-8958 |
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| Description | Sulfo-NHS-SS-Biotin is a water-soluble, NHS-ester biotinylation reagent | |||||
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Quality Control & MSDS
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Chemical structure
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