Sulfo-NHS-LC-Biotin Kit
Sulfo-NHS-LC-Biotin is a water-soluble biotinylation reagent designed for labeling primary amines (–NH₂), such as those found in antibodies, proteins, peptides, or other biomolecules. The reagent consists of a biotin moiety linked by a long-chain spacer arm to N-hydroxysulfosuccinimide (Sulfo-NHS), which readily reacts with amino groups to form stable amide bonds. The formed biotinylated conjugates retain biological activity and affinity toward streptavidin or avidin. In biochemical research, this biotinylation method is utilized for protein-protein interactions, immunoassays, affinity chromatography purification, cell-surface labeling, and immunofluorescence studies requiring subsequent detection or enrichment via avidin-based probes or resins. The included reagents facilitate rapid labeling and purification steps under aqueous conditions without dialysis.
| Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody | |||
| Streptavidin | 10 mg | Sulfo-NHS-LC-Biotin | 25 mg |
| HABA Solution | 1 mL | PBS Pack (makes 1 L) | 1 pack |
| Sephadex G-25 in PD-10 Desalting Columns | 8.3 mL, 10 columns | ||
| Store biotin and streptavidin reagents at -20°C. Store remaining kit components at 4°C. | |||
| Label or Dye: | Biotin | Product Size: | 10 reactions |
| Labeling Scale: | 1-10 mg | Labeling Target: | Proteins (General), Antibodies (General) |
• Protein labeling—biotin antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Antibody labeling—biotin antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—biotin only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Medium length—spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass
Sulfo-NHS-LC-Biotin is one of three very similar Reagents that are water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three Sulfo-NHS-ester reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Sulfo-NHS-LC-Biotin is offered in several package sizes and as complete protein labeling kits.
