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Sulfo-NHS-Biotin
Amine-reactive biotinyltation reagent, water soluble

Sulfo-NHS-Biotin

Catalog No. A8001
Size Price Stock Qty
100mg $75.00 In stock
1g $380.00 In stock

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Sample solution is provided at 25 µL, 10mM.

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Chemical structure

Sulfo-NHS-Biotin

Related Biological Data

Sulfo-NHS-Biotin

Biological Activity

Description Water-soluble biotinyltation reagent to attach biotin to primary amines
Targets            
IC50            

Protocol

Biotinylation method [1]:

Sample

THUMPa protein

Preparation method

Soluble in water, DMSO or DMF.

Reaction Conditions

2mM, room temperature for 30min

Applications

Sulfo-NHS-biotin reagents were dissolved at 2 mM in a 50-mM K2HPO4/KH2PO4 buffer (pH 7.5) containing 50 mM of NaCl. They were immediately used in separated assays to modify 1.66 nmol of THUMPa protein. The labeling reactions were incubated 30 min at room temperature. The samples were then dialyzed for 15 min against a 50-mM K2HPO4/KH2PO4 buffer (pH 7.5) containing 50 mM of NaCl. TTotal biotin covalently bound to proteins was determined by an avidin-binding assay.

References:

[1]. Guillaume Gabant, Julie Augier and Jean Armengaud. Assessment of solvent residues accessibility using three Sulfo-NHS-biotin reagents in parallel: application to footprint changes of a methyltransferase upon binding its substrate. J. Mass Spectrom. 2008; 43: 360–370.

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Chemical Properties

Cas No. 119616-38-5 SDF Download SDF
Synonyms Sulfo-NHS Biotin
Chemical Name sodium;1-[5-[(3aS,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate
Canonical SMILES C1C(C(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]
Formula C14H18N3NaO8S2 M.Wt 443.4
Solubility >22.2mg/mL in DMSO Storage Desiccate at -20°C
General tips Sulfo-NHS-Biotin is moisture-sensitive. If the vial of reagent has been stored cold, fully equilibrate vial to room temperature before opening to avoid moisture condensation inside the container. Do not prepare stock solutions for storage, and dissolve the biotin reagent immediately before use. Avoid buffers containing primary amines (e.g., Tris or glycine) as these will compete with the reaction.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

Features

• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains or the N-terminal-amine
• Solubility—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Very short—spacer arm is 13.5 angstroms; it consists of the native biotin valeric acid group only

Principle

Background

Sulfo-NHS-Biotin (N-Hydroxysulfosuccinimidobiotin) is an amine-reactive biotinylation reagent. The most common amine-reactive biotinylation reagents can be divied into two basic types, N-hydroxysuccinimide (NHS) esters and carboxylates.  Generally, NHS esters biotinylation reagent contains a reactive NHS ring structure that reacts with an amine on the carbonyl group of a protein or other molecules through nucleophilic attack subsequently forming a stable amide linkage and releasing the NHS group. Due to the insolubility of NHS-biotin in aqueous environments, sulfo-NHS-biotin, a water-soluble analog of NHS-biotin, has been developed by attaching a negatively charged sulfonate group on the NHS ring structure, which can be added directly to aqueous reactions without the need for organic solvent dissolution.

Reference

Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson  (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL).  Academic Press  (an imprint of Elsevier):  London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.