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Sulfo-NHS-Biotin

Catalog No.
A8001
Sulfo-NHS-Biotin
Grouped product items
SizePriceStock Qty
25mg
$60.00
In stock
50mg
$90.00
In stock
100mg
$120.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

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Background

Sulfo-NHS-Biotin is a water-soluble biotinylation reagent frequently utilized in biochemical research for labeling proteins and other biomolecules. Chemically, it contains an N-hydroxysulfosuccinimide (Sulfo-NHS) ester group, allowing for covalent linkage through amine-reactive chemistry. Upon interaction with primary amines on lysine residues or peptide N-termini, the Sulfo-NHS ester undergoes nucleophilic attack, forming stable amide bond conjugates and releasing an NHS derivative. Its solubility in aqueous solutions facilitates direct addition to biological samples without prior dissolution in organic solvents. Common research applications include protein purification, affinity chromatography, immunoprecipitation assays, and detection in protein interaction studies.

Reference

Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson  (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL).  Academic Press  (an imprint of Elsevier):  London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.

Product Citation

Features

• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains or the N-terminal-amine
• Solubility—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Very short—spacer arm is 13.5 angstroms; it consists of the native biotin valeric acid group only

Chemical Properties

Physical AppearanceA solid
StorageDesiccate at -20°C
The product is not stable in solution, please dissolve it immediately before use.
M.Wt443.4
Cas No.119616-38-5
FormulaC14H18N3NaO8S2
SynonymsSulfo-NHS Biotin
Solubility≥22.17 mg/mL in DMSO; insoluble in EtOH; ≥16.8 mg/mL in H2O with ultrasonic
Chemical Namesodium;1-[5-[(3aS,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate
SDFDownload SDF
Canonical SMILESC1C(C(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Biotinylation method [1]:

Sample

THUMPa protein

Preparation method

Soluble in water, DMSO or DMF.

Reaction Conditions

2 mM, room temperature for 30 min

Applications

Sulfo-NHS-biotin reagents were dissolved at 2 mM in a 50-mM K2HPO4/KH2PO4 buffer (pH 7.5) containing 50 mM of NaCl. They were immediately used in separated assays to modify 1.66 nmol of THUMPa protein. The labeling reactions were incubated 30 min at room temperature. The samples were then dialyzed for 15 min against a 50-mM K2HPO4/KH2PO4 buffer (pH 7.5) containing 50 mM of NaCl. TTotal biotin covalently bound to proteins was determined by an avidin-binding assay.

References:

[1]. Guillaume Gabant, Julie Augier and Jean Armengaud. Assessment of solvent residues accessibility using three Sulfo-NHS-biotin reagents in parallel: application to footprint changes of a methyltransferase upon binding its substrate. J. Mass Spectrom. 2008; 43: 360–370.

Biological Activity

Description Water-soluble biotinyltation reagent to attach biotin to primary amines
Targets            
IC50            

Quality Control

Quality Control & MSDS

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Chemical structure

Sulfo-NHS-Biotin

Related Biological Data

Sulfo-NHS-Biotin

Related Biological Data

Sulfo-NHS-Biotin

Related Biological Data

Sulfo-NHS-Biotin