NHS-LC-Biotin

mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail

Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.

Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody

Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay

SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.

Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NHS-LC-biotin (succinimidyl-6-(biotinamindo)hexanoate), also known as NHS-X-biotin is a derivative of D-biotin, a amine-reactive biotinylation agent, that contains a spacer arm off the valeric acid side chain of D-biotin with an NHS ester group at its end. The NHS ester group at the end of NHS-LC-biotin covalently binds to amine groups in proteins and other molecules forming a stable amide linkage and releasing the NHS group. The 6-aminocaproic acid spacer of NHS-LC-biotin greatly increases the length between a covalently modified molecule and the bicyclic biotin rings leading to a better binding potential for avidin or streptavidin probes. NHS-LC-biotin is insoluble in aqueous environments requiring the dissolution of organic solvents prior to the addition to a buffered reaction.
Reference
Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
- 1. Cong H, Wang K, et al. "Tuning the Brightness and Photostability of Organic Dots for Multivalent Targeted Cancer Imaging and Surgery." ACS Nano. 2020;14(5):5887-5900. PMID:32356972
- 2. JIUHE ZHU. "The regulation of neuronal excitability by epilepsy-associated gene Nedd4-2." University of Illinois. Urbana-Champaign, 2019
- 3. Kamperman T, Koerselman M, et al. "Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs." Nat Commun. 2019 Sep 25;10(1):4347. PMID:31554812
- 4. Zhang X, Qiu M, et al. "Autologous Red Blood Cell Delivery of Betamethasone Phosphate Sodium for Long Anti-Inflammation." Pharmaceutics. 2018 Dec 18;10(4). pii: E286. PMID:30567356
- 5. Zhu J, Lee KY, et al. "Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors." PLoS Genet. 2017 Feb 17;13(2):e1006634. PMID:28212375
• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Intracellular labeling—membrane-permeable, can be used to label inside cells
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the N-terminal-amine
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Solubility—water insoluble, must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Medium length—spacer arm is 22.4 angstroms; it consists of the biotin valeric acid group extended by a 6-atom chain
Storage | Store at -20°C |
M.Wt | 454.54 |
Cas No. | 72040-63-2 |
Formula | C20H30N4O6S |
Synonyms | Succinimidyl 6-(biotinamido)hexanoate |
Solubility | ≥22.73 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
Chemical Name | (2,5-dioxopyrrolidin-1-yl) 6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoate |
SDF | Download SDF |
Canonical SMILES | C1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3 |
Shipping Condition | Ship with blue ice, or upon other requests. |
General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon. |
Biotinylation method [1]: | |
Sample |
S. aureus |
Preparation method |
Soluble in DMSO or DMF. |
Reaction Conditions |
60 mg/ml, room temperature for 1 h. |
Applications |
Fixed cell suspensions of S. aureus were diluted to a 2% cell suspension in 0.2 M NaHCO3, pH 8.3. NHS-LC-biotin freshly dissolved in DMSO at a concentration of 60 mg/ml, was added to the cell suspension subsequently. After gentle tumble mixing for 60min at room temperature, the reaction was stopped by removal of excess biotin via gel filtration over a PD-10 column and washed extensively in 0.01 mM Tris-buffered 0.15 M NaCl (TBS). Successful protein biotinylation was examining their reactivity with alkaline phosphatase-conjugated streptavidin(AP-STRAV). AP-STRAV binding was detected as a function of pNPP hydrolysis. |
References: [1]. TRUC NGUYEN, BERHANE GHEBREHIWET, AND ELLINOR I. B. PEERSCHKE. Staphylococcus aureus Protein A Recognizes Platelet gC1qR/p33: a Novel Mechanism for Staphylococcal Interactions with Platelets. INFECTION AND IMMUNITY 2000, p. 2061–2068. |
Description | NHS-LC-Biotin is a amine-reactive biotinylation agent | |||||
Targets | ||||||
IC50 |
Quality Control & MSDS
- View current batch:
Chemical structure

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