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NHS-LC-Biotin

Catalog No.
A8004
NHS-LC-Biotin
Grouped product items
SizePriceStock Qty
100mg
$60.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

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Background

NHS-LC-Biotin, also known as NHS-X-biotin, is a biotinylation reagent designed to tag proteins or biomolecules through covalent conjugation with primary amines. Structurally, it consists of a biotin moiety connected via a hexanoic acid spacer arm terminated with an N-hydroxysuccinimide (NHS) ester group. The NHS ester reacts selectively with free amine groups on target proteins or other molecules, forming stable amide bonds to yield biotinylated conjugates accessible to subsequent detection and purification steps via streptavidin or avidin affinity. The hexanoic acid spacer enhances spatial separation between the biotin residue and the target molecule, improving binding accessibility in applications including immunoblotting, affinity chromatography, protein localization, and biomolecule labeling in biochemical research.

Reference

Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson  (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL).  Academic Press  (an imprint of Elsevier):  London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.

Product Citation

Features

• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Intracellular labeling—membrane-permeable, can be used to label inside cells
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the N-terminal-amine
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Solubility—water insoluble, must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Medium length—spacer arm is 22.4 angstroms; it consists of the biotin valeric acid group extended by a 6-atom chain

Chemical Properties

StorageStore at -20°C
M.Wt454.54
Cas No.72040-63-2
FormulaC20H30N4O6S
SynonymsSuccinimidyl 6-(biotinamido)hexanoate
Solubility≥22.73 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O
Chemical Name(2,5-dioxopyrrolidin-1-yl) 6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoate
SDFDownload SDF
Canonical SMILESC1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Biotinylation method [1]:

Sample

S. aureus

Preparation method

Soluble in DMSO or DMF.

Reaction Conditions

60 mg/ml, room temperature for 1 h.

Applications

Fixed cell suspensions of S. aureus were diluted to a 2% cell suspension in 0.2 M NaHCO3, pH 8.3. NHS-LC-biotin freshly dissolved in DMSO at a concentration of 60 mg/ml, was added to the cell suspension subsequently. After gentle tumble mixing for 60min at room temperature, the reaction was stopped by removal of excess biotin via gel filtration over a PD-10 column and washed extensively in 0.01 mM Tris-buffered 0.15 M NaCl (TBS). Successful protein biotinylation was examining their reactivity with alkaline phosphatase-conjugated streptavidin(AP-STRAV). AP-STRAV binding was detected as a function of pNPP hydrolysis.

References:

[1]. TRUC NGUYEN, BERHANE GHEBREHIWET, AND ELLINOR I. B. PEERSCHKE. Staphylococcus aureus Protein A Recognizes Platelet gC1qR/p33: a Novel Mechanism for Staphylococcal Interactions with Platelets. INFECTION AND IMMUNITY 2000, p. 2061–2068.

Biological Activity

Description NHS-LC-Biotin is a amine-reactive biotinylation agent
Targets            
IC50            

Quality Control

Chemical structure

NHS-LC-Biotin

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