Sulfo-NHS-LC-Biotin
Sulfo-NHS-LC-Biotin (sulfosuccinimidyl-6-(biotinamido) hexanoate) is a water-soluble reagent for covalently labeling primary amines (especially on proteins/peptides) via stable amide bonds (releasing NHS), with a negatively charged sulfonate group enabling aqueous use without organic solvents. Its 22.4 Å hexanoate spacer enhances accessibility to sterically hindered sites, compared to shorter linker derivatives (A8001). It is widely used in molecular biology/biochemical assays for detecting, purifying, or immobilizing amine-containing biomolecules via biotin-avidin/streptavidin systems.
Reference
Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
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• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the N-terminal-amine
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Medium length—spacer arm is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass
| Storage | Store at -20°CThe product is not stable in solution, please dissolve it immediately before use. |
| M.Wt | 556.6 |
| Cas No. | 127062-22-0 |
| Formula | C20H29N4NaO9S2 |
| Synonyms | Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt |
| Chemical Name | 2,5-dioxo-1-[6-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoylamino]hexanoyloxy]pyrrolidine-3-sulfonic acid;sodium |
| SDF | Download SDF |
| Canonical SMILES | C1C(C(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)O.[Na] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Biotinylation method [1]: | |
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Sample |
Cardiomyocytes |
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Preparation method |
Soluble in Water, DMSO or DMF. |
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Reaction Conditions |
0.5 mg/ml, 37ºC for 2 h |
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Applications |
Sulfo-NHS-LC-Biotin was dissolved in PBS at a concentration of 0.5 mg/ml. then it was incubated with cardiomyocytes at 37ºC for 2 h. After that the cells were washed twice in cold PBS with 100 mM glycine and twice in cold PBS alone to remove the excess biotin reagent. To capture biotinylated proteins, 100 μl of streptavidin agarose resin were incubated with each 100 μg of samples for 16 hours at 4ºC. Captured proteins were eluted at 60ºC for 30 min with Laemmli 2X sample buffer which contain 40 mg/mL of dithiothreitol and they were analyzed by Western blot with primary antibodies. |
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References: [1]. Charlotte Lorin, Isabelle Vögeli, Ernst Niggli. Dystrophic cardiomyopathy - role of TRPV2 channels in stretch-induced cell damage. Cardiovascular Research, 2015. |
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| Description | Sulfo-NHS-LC-Biotin is an intermediate length, water-soluble biotinyltation reagent used to attach biotin to primary amines. | |||||
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Quality Control & MSDS
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