Streptavidin – FITC
Catalog No.
K1081
Used in fluorescent detection of biotin conjugated antibodies, proteins or other molecules
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Streptavidin is a 52,800 dalton homotetramer protein. It has an extraordinarily high affinity for biotin, one molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Streptavidin- FITC is used in fluorescent staining of biotinylated antibodies, proteins, nucleic acids or other molecules in immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), in situ hybridization (ISH), or flow cytometry. FITC is excited maximally at 488 nm, emits maximally at ~520 nm.
| Physical Appearance | Solution. Streptavidin-FITC is supplied in phosphate buffered saline (PBS), pH 7.4, containing 1% BSA, 0.1% sodium azide. |
| Storage | Store at 2-8°C. Light exposure should be avoided. Do not freeze. |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
0.5 mg/mL
Recommended Dilution:
1:20 to 1:50 dilution with PBS containing 10% normal goat serum. Working dilution for specific application should be determined by individual investigator to obtain the best conditions.
Recommended Dilution:
1:20 to 1:50 dilution with PBS containing 10% normal goat serum. Working dilution for specific application should be determined by individual investigator to obtain the best conditions.
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