Sulfo-NHS-SS-11-Biotin
Sulfo-NHS-SS-11-biotin is a long-chain cleavable amine-reactive biotinylation reagent. The presence of the negatively charged sulfonate group in the chemical structure of sulfo-NHS-SS-11-biotin makes it a water-soluble biotinylation reagent that can be directly added to aqueous reactions without prior dissolution of organic solvents. Although no prior dissolution is required, an aqueous stock solution of sulfo-NHS-SS-11-biotin must be prepared rapidly and used immediately in case of the occurrence of hydrolysis of the active ester. Sulfo-NHS-SS-11-biotin has been used to react with amine-containing proteins and other molecules forming a complex which further interacts with avidin or streptavidin probes and to purify targeted molecules using affinity chromatography on a column of immobilized avidin or streptavidin.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 677.77 |
| Formula | C22H32N5NaO10S4 |
| Solubility | insoluble in EtOH; ≥48.9 mg/mL in H2O; ≥49.2 mg/mL in DMSO |
| Chemical Name | sodium 2,5-dioxo-1-((3-((3-oxo-3-((2-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethyl)amino)propyl)disulfanyl)propanoyl)oxy)pyrrolidine-3-sulfonate |
| SDF | Download SDF |
| Canonical SMILES | O=C1N[C@]2([H])[C@]([C@H](CCCCC(NCCNC(CCSSCCC(ON3C(CC(S(=O)(O[Na])=O)C3=O)=O)=O)=O)=O)SC2)([H])N1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
