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Catalog No.
Amine-reactive biotinyltation reagent, water soluble
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Sulfo-NHS-Biotin (N-Hydroxysulfosuccinimidobiotin) is an amine-reactive biotinylation reagent. The most common amine-reactive biotinylation reagents can be divied into two basic types, N-hydroxysuccinimide (NHS) esters and carboxylates. Generally, NHS esters biotinylation reagent contains a reactive NHS ring structure that reacts with an amine on the carbonyl group of a protein or other molecules through nucleophilic attack subsequently forming a stable amide linkage and releasing the NHS group. Due to the insolubility of NHS-biotin in aqueous environments, sulfo-NHS-biotin, a water-soluble analog of NHS-biotin, has been developed by attaching a negatively charged sulfonate group on the NHS ring structure, which can be added directly to aqueous reactions without the need for organic solvent dissolution.


Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson  (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL).  Academic Press  (an imprint of Elsevier):  London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.

Product Citation


• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains or the N-terminal-amine
• Solubility—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Very short—spacer arm is 13.5 angstroms; it consists of the native biotin valeric acid group only

Chemical Properties

Physical AppearanceA solid
StorageDesiccate at -20°C
The product is not stable in solution, please dissolve it immediately before use.
Cas No.119616-38-5
SynonymsSulfo-NHS Biotin
Solubility≥22.17 mg/mL in DMSO; insoluble in EtOH; ≥59.9 mg/mL in H2O with ultrasonic
Chemical Namesodium;1-[5-[(3aS,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate
SDFDownload SDF
Canonical SMILESC1C(C(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.


Biotinylation method [1]:


THUMPa protein

Preparation method

Soluble in water, DMSO or DMF.

Reaction Conditions

2 mM, room temperature for 30 min


Sulfo-NHS-biotin reagents were dissolved at 2 mM in a 50-mM K2HPO4/KH2PO4 buffer (pH 7.5) containing 50 mM of NaCl. They were immediately used in separated assays to modify 1.66 nmol of THUMPa protein. The labeling reactions were incubated 30 min at room temperature. The samples were then dialyzed for 15 min against a 50-mM K2HPO4/KH2PO4 buffer (pH 7.5) containing 50 mM of NaCl. TTotal biotin covalently bound to proteins was determined by an avidin-binding assay.


[1]. Guillaume Gabant, Julie Augier and Jean Armengaud. Assessment of solvent residues accessibility using three Sulfo-NHS-biotin reagents in parallel: application to footprint changes of a methyltransferase upon binding its substrate. J. Mass Spectrom. 2008; 43: 360–370.

Biological Activity

Description Water-soluble biotinyltation reagent to attach biotin to primary amines

Quality Control

Quality Control & MSDS

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Chemical structure


Related Biological Data


Related Biological Data


Related Biological Data