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Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)

Catalog No.
Equivalent to Sigma P9599, for use with plant cell and tissue extracts to increase protein stability.
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Tel: +1-832-696-8203

Email: [email protected]

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Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Protease inhibitor cocktail is used in plant cell lysates or tissue extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates.

This protease inhibitor cocktail contains individual components, including AEBSF, 1,10-Phenanthroline, Bestatin, E-64, Leupeptin and Pepstatin A with a broad specificity for cysteine, serine, aspartic, metalloproteases, and aminopeptidases. This protease inhibitor cocktail has been optimized and tested for plant cells and tissue extracts. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.

Quality Control

Quality Control & DataSheet

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Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as plant cell lysates or tissue extracts) before assaying.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.


Stored at -20°C, and stable for at least 12 months.

Components and Storage

Catalog No. Product Name Summary Targets CAS Number Smiles
A2573 AEBSF.HCl Serine protease inhibitor Proteases|Serine Protease 30827-99-7 C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl
A2575 Bestatin Aminopeptidase inhibitor Proteases|Aminopeptidase 58970-76-6 CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O
A2576 E-64 Cysteine protease inhibitor,irriversible Proteases|Cathepsin 66701-25-5 CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O
A2570 Leupeptin, Microbial Inhibitor of serine and cysteine proteases Proteases|Serine Protease 103476-89-7 O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H]
A2571 Pepstatin A Aspartic proteinases inhibitor Proteases|Other Proteases 26305-03-3 CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O
B7854 o-Phenanthroline metalloprotease inhibitor Proteases|Other Proteases 66-71-7 C(C=CC=N1)(C=C2)=C1C3=C2C=CC=N3
Stored at -20°C, and stable for at least 12 months.