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Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)

Catalog No.
Equivalent to Sigma P8215, for use with fungal and yeast extracts to increase protein stability.
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Email: [email protected]

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Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Protease inhibitor cocktail is used with fungal and yeast extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates.

His protease inhibitor cocktail contains individual components, including AEBSF, 1,10-Phenanthroline, E-64 and Pepstatin A with a broad specificity for cysteine, serine, aspartic, and metalloproteases. This cocktail has been optimized and tested for fungal and yeast cell use. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.

Quality Control

Quality Control & DataSheet

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Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as yeast extracts) before assaying.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.


Stored at -20°C, and stable for at least 12 months.

Components and Storage

Catalog No. Product Name Summary Targets CAS Number Smiles
B7854 o-Phenanthroline metalloprotease inhibitor Proteases|Other Proteases 66-71-7 C(C=CC=N1)(C=C2)=C1C3=C2C=CC=N3
A2573 AEBSF.HCl Serine protease inhibitor Proteases|Serine Protease 30827-99-7 C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl
A2576 E-64 Cysteine protease inhibitor,irriversible Proteases|Cathepsin 26305-03-3 CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O
A2571 Pepstatin A Aspartic proteinases inhibitor Proteases|Other Proteases 103476-89-7 O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H]
Stored at -20°C, and stable for at least 12 months.