Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)
Cell crude extracts contain various endogenous enzymes, such as phosphatases and proteases, capable of degrading proteins present in the extracts.The optimal strategy to enhance intact protein yield is adding specific inhibitors targeting these enzymes.This Protease Inhibitor Cocktail is used in fungal and yeast extracts to improve protein stability by inhibiting proteases responsible for degrading both phosphorylated and non-phosphorylated protein substrates.
This Protease Inhibitor Cocktail consists of individual compounds including AEBSF, 1,10-Phenanthroline, E-64, and Pepstatin A, which provide broad-spectrum, specific inhibition of cysteine proteases, serine proteases, aspartic proteases, and metalloproteases. It is optimized and tested for fungal and yeast extracts. This cocktail is available as a ready-to-use solution dissolved in DMSO.
- 1. Sohail Ahmad, Siying Zhang, et al. "A conserved thumb domain insertion in DNA polymerase epsilon supports processive DNA synthesis." Nucleic Acids Res. 2025 Feb 27;53(5):gkaf190. PMID: 40105244
- 2. Yunfei Zhang, Gang Liu, et al. "SUN5 interacts with nuclear membrane LaminB1 and cytoskeletal GTPase Septin12 mediating the sperm head-and-tail junction." Mol Hum Reprod. 2024 Jun 26;30(7):gaae022. PMID: 38870534
- 3. Yuze Yan, Miaomiao Tian, et al. "ASH1L haploinsufficiency results in autistic-like phenotypes in mice and links Eph receptor gene to autism spectrum disorder." Neuron. 2022 Jan 20;S0896-6273(21)01090-4. PMID: 35081333
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as yeast extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| B7854 | o-Phenanthroline | metalloprotease inhibitor | Proteases|Other Proteases | 66-71-7 | C(C=CC=N1)(C=C2)=C1C3=C2C=CC=N3 |
| A2573 | AEBSF.HCl | Serine protease inhibitor | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl |
| A2576 | E-64 | Cysteine protease inhibitor,irriversible | Proteases|Cathepsin | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O |
| A2571 | Pepstatin A | Aspartic proteinases inhibitor | Proteases|Other Proteases | 103476-89-7 | O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] |
| Stored at -20°C, and stable for at least 12 months. | |||||
