Prestained Protein Marker (Triple color, EDTA free, 10-250 kDa)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The Prestained Protein Marker (Triple color, EDTA free, 10-250 kDa) is a prestained mixture of ten recombinant proteins ranging from 10 kDa to 250 kDa. Three different chromophores are bound to the proteins, 9 bands with blue, one red at 70 kDa and one green at 25 kDa. The Prestained Protein Marker is applied for observing protein separation during SDS-PAGE electrophoresis, verification of western transfer efficiency on membranes, and identifying the size of proteins. The marker can also be used in fluorescent imaging of membranes.
Prestained Protein Marker obtained increased brightness providing visual robustness throughout western blotting workflow, to the benefit of identifying target proteins on the gel or membrane, monitoring gel electrophoresis and confirming transfer quality.
The buffer of the marker doesn’t contain EDTA which means it can be used in Phosbind SDS-PAGE electrophoresis.
The recommended volume for 1mm mini-gel is 3-5 μl. Large size gels need more volume.
Quality Control & MSDS
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Components and Storage
|Prestained Protein Marker (Triple color, EDTA free, 10-250 KDa)||250uL|
|Prestained Protein Marker (Triple color, EDTA free, 10-250 kDa)||2x250uL|
Store the marker at -20 ℃. The marker can be stored at 4 ℃ for several days.
Prestained Protein Marker can be directly loaded, additional loading buffer and heat incubation is not required
Recombinant proteins in the components with no detectable protease contaminating activities
Prestained Protein Marker can be widely Used with all common membranes: PVDF, nylon and nitrocellulose.