ECL Chemiluminescent Substrate Detection Kit (Hypersensitive)
ECL Chemiluminescent Substrate Detection Kit (Hypersensitive), using HRP-mediated oxidation to generate chemiluminescent signals, is for immunoblotting to detect antigens on nitrocellulose/PVDF membranes, with high sensitivity (low picogram range) for low-abundance proteins. Its signals persist for 6-8 hours under optimal conditions, and the mixed working reagent is stable for 24 hours. It has an extended room-temperature shelf-life, and compared to conventional kits (e.g., from T and M), it shows lower background and longer signal duration.
Product Features
（1) Enhanced chemiluminescent substrate for horseradish peroxidase (HRP).
（2) Low picogram sensitivity - Detects low picogram protein bands on nitrocellulose or PVDF membranes.
（3) Long signal duration - Under optimized conditions, substrate-incubated blots can consistently output a detectable light signal for 6 to 8 hours.
（4) Stable reagent - The working solution remains stable for 24 hours; The kit can be stored at room temperature for up to 1 year.
（5) Cost-effective - Optimized for diluted antibody concentrations.
- 1. Chen Li, Yike Li, et al. "Unveiling the neuroprotective power of mitochondrial transfer in orofacial inflammatory pain through ER membrane remodeling." Cell Reports Volume 45, Issue 1, 27 January 2026, 116809. PMID: 41538328
- 2. Shuang Lu, Lewen Wang, et al. "As a novel ferroptosis inhibitor, primaquine alleviates I/R-induced retinal neuron death via increasing GSTA1 activity." Biomedicine & Pharmacotherapy Volume 195, February 2026, 118973. PMID: 41529513
- 3. Xingpeng Wang, Lian Zhong, et al. "Target Validation and Drug Discovery for TNBC: Targeting the Lin28B/Let-7/PBK with the Ponicidin." European Journal of Medicinal Chemistry Volume 304, 15 February 2026, 118474. PMID: 41406550
- 4. Jingtian Mu, Tingpei Ye, Junjiang Liu. "CAFs-secreted Fatty Acids Fuel Oral Cancer Progression via Lipid Raft Formation." Arch Oral Biol . 2025 Aug 15:179:106377. PMID: 40840065
- 5. Qi Zhang, Ruiqi Wang, et al. "A humanized Gs-coupled DREADD for circuit and behavior modulation." Front Cell Neurosci. 2025 Apr 9;19:1577117. PMID: 40271540
- 6. Yan-Yun Liu, Zheng-Ao Li, et al. "TCM theory-inspired discovery of DNJ-flavonoid conjugates as broad-spectrum anti-SARS-CoV-2 agents by primarily targeting ER-associated glycoprotein folding process." Eur J Med Chem. 2025 Jun 5:290:117582. PMID: 40168909
- 7. Jiajing Cai, Bei Xia, et al. "Site-Specific DNA methylation detection using nonlinear hybridization chain reaction." Microchemical Journal. Volume 212, May 2025, 113522
- 8. ZHINA WU, RUI LIU, et al. "An enzymatic cleavage-triggered minimally invasive nanosensor for urine-based detection of early atherosclerosis." SCIENCE ADVANCES 14 Mar 2025 Vol 11, Issue 11
- 9. Weiyun Wu, Xiaowen Li, et al. "METTL14 regulates inflammation in ulcerative colitis via the lncRNA DHRS4-AS1/miR-206/A3AR axis." Cell Biol Toxicol 40, 95 (2024)
Related Biological Data
Related Biological Data
Related Biological Data
| Components | 100 mL | 500 mL |
| ECL Chemiluminescent Substrate Detection Kit (Hypersensitive)-A | 50 mL | 250 mL |
| ECL Chemiluminescent Substrate Detection Kit (Hypersensitive)-B | 50 mL | 250 mL |
Store the components dry at 4 °C and protect from light for 12 months. | ||
