ECL Chemiluminescent Substrate Detection Kit (Hypersensitive)
The ECL Chemiluminescent Substrate Detection Kit (Hypersensitive) provides a bright, high-intensity signal for immunoblotting detection with HRP-labeled antibodies. It facilitates low-picogram to femtogram-level detection (based on HRP-conjugate concentration) in Western blots. This ECL substrate is compatible with a variety of membrane types, blocking solutions, and a broad range of antibody dilutions, providing superior performance, versatility, and cost-effectiveness to meet WB needs.
Product features:
(1) Enhanced chemiluminescent substrate designed for horseradish peroxidase (HRP).
(2) Low picogram-level sensitivity—detect protein bands at low picogram levels on NC or PVDF membranes.
(3) Long signal duration—Optimally incubated blots maintain a detectable chemiluminescent signal for 6 to 8 hours.
(4) Stable reagent—Working solution remains stable for 24 hours; kit stability reaches up to 1 year at RT.
(5) Cost-efficient—Optimized for use with diluted Ab conc.
Product Features
(1) Enhanced chemiluminescent substrate for horseradish peroxidase (HRP).
(2) Low picogram sensitivity - Detects low picogram protein bands on nitrocellulose or PVDF membranes.
(3) Long signal duration - Under optimized conditions, substrate-incubated blots can consistently output a detectable light signal for 6 to 8 hours.
(4) Stable reagent - The working solution remains stable for 24 hours; The kit can be stored at room temperature for up to 1 year.
(5) Cost-effective - Optimized for diluted antibody concentrations.
- 1. ZHINA WU, RUI LIU, et al. "An enzymatic cleavage-triggered minimally invasive nanosensor for urine-based detection of early atherosclerosis." SCIENCE ADVANCES 14 Mar 2025 Vol 11, Issue 11
- 2. Weiyun Wu, Xiaowen Li, et al. "METTL14 regulates inflammation in ulcerative colitis via the lncRNA DHRS4-AS1/miR-206/A3AR axis." Cell Biol Toxicol 40, 95 (2024).
Components | 100 mL | 500 mL |
ECL Chemiluminescent Substrate Detection Kit (Hypersensitive)-A | 50 mL | 250 mL |
ECL Chemiluminescent Substrate Detection Kit (Hypersensitive)-B | 50 mL | 250 mL |
Store the components dry at 4 °C and protect from light for 12 months. |