ECL Chemiluminescent Substrate Detection Kit (Hypersensitive)
ECL Chemiluminescent Substrate Detection Kit (Hypersensitive), using HRP-mediated oxidation to generate chemiluminescent signals, is for immunoblotting to detect antigens on nitrocellulose/PVDF membranes, with high sensitivity (low picogram range) for low-abundance proteins. Its signals persist for 6-8 hours under optimal conditions, and the mixed working reagent is stable for 24 hours. It has an extended room-temperature shelf-life, and compared to conventional kits (e.g., from T and M), it shows lower background and longer signal duration.
Product Features
(1) Enhanced chemiluminescent substrate for horseradish peroxidase (HRP).
(2) Low picogram sensitivity - Detects low picogram protein bands on nitrocellulose or PVDF membranes.
(3) Long signal duration - Under optimized conditions, substrate-incubated blots can consistently output a detectable light signal for 6 to 8 hours.
(4) Stable reagent - The working solution remains stable for 24 hours; The kit can be stored at room temperature for up to 1 year.
(5) Cost-effective - Optimized for diluted antibody concentrations.
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Components | 100 mL | 500 mL |
ECL Chemiluminescent Substrate Detection Kit (Hypersensitive)-A | 50 mL | 250 mL |
ECL Chemiluminescent Substrate Detection Kit (Hypersensitive)-B | 50 mL | 250 mL |
Store the components dry at 4 °C and protect from light for 12 months. |