Phosphatase Inhibitor Cocktail 2 (100X in ddH2O)
Many cellular processes are regulated by reversible phosphorylation of proteins, and approximately 30% of proteins may become phosphorylated during their lifetime. Cellular crude extracts contain numerous endogenous enzymes, such as phosphatases and proteases, which can degrade and modify proteins present in the extract. Addition of inhibitors for these enzymes is the optimal approach for maximizing intact protein yield.
Phosphatase Inhibitor Cocktail 2 inhibits tyrosine protein phosphatases, acid phosphatases, and alkaline phosphatases. Phosphatase Inhibitor Cocktail 2 has been optimized and tested in cell extracts from multiple animal tissues. This Phosphatase Inhibitor Cocktail 2 contains Sodium orthovanadate, Sodium molybdate, Sodium tartrate, Imidazole, and Sodium Fluoride. It is supplied as a ready-to-use solution in ddH2O.
- 1. Yufeng Zhang, Jie Wang, et al. "An ancient regulatory variant of ACSF3 influences the coevolution of increased human height and basal metabolic rate via metabolic homeostasis." Cell Genom. 2025 May 21;5(6):100855 PMID: 40403731
- 2. Fengwu Zhang, Xiufang Xiong, et al. "RHEB neddylation by the UBE2F-SAG axis enhances mTORC1 activity and aggravates liver tumorigenesis." EMBO J. 2025 Jan 6 PMID: 39762645
- 3. Qiuyu BaoNing Wan, et al. "Subcellular Proteomic Mapping of Lysine Lactylation" Journal of the American Society for Mass SpectrometryVol 35/Issue 12 November 21, 2024
- 4. Aakarsha Rao, Milan S Stosic, et al. "Targeted inhibition of BET proteins in HPV16-positive head and neck squamous cell carcinoma reveals heterogeneous transcriptional responses." Front Oncol. 2024 Sep 5:14:1440836. PMID: 39301555
- 5. Chang Shao, Shuo Tang, et al. "Genetic code expansion reveals site-specific lactylation in living cells reshapes protein function." bioRxiv. September 15, 2024.
- 6. Debra Toiber, Daniel Stein, et al. "SIRT6 regulates protein synthesis and folding through nucleolar remodeling." Research Square. 28 May, 2024
- 7. Xiaojing Zhang, et al. "The effects of restraint stress on ceramide metabolism disorders in the rat liver: the role of CerS6 in hepatocyte injury." Lipids Health Dis. 2024 Mar 2;23(1):68. PMID: 38431645
- 8. Sung-Suk Suh, et al. "CREB5 promotes the proliferation and self-renewal ability of glioma stem cells." Cell Death Discov. 2024 Feb 28;10(1):103. PMID: 38418476
- 9. Aakarsha Rao, Zijian Ni, et al. "Targeted inhibition of BET proteins in HPV-16 associated head and neck squamous cell carcinoma reveals heterogeneous transcription response." bioRxiv. 2023 Oct 4:2023.10.02.560587. PMID: 37873389
- 10. Payal Deepak, Praveen Kumar, et al. "The triglyceride catabolism regulated by a serine/threonine protein phosphatase, Smek1, is required for development and plant infection inMagnaporthe oryzae." Int J Pharm. 2023 Jul 25:642:123160. PMID: 37379892
- 11. Tian Y, Wan N, et al. "Chemoproteomic mapping of the glycolytic targetome in cancer cells." Nature Chemical Biology, 2023: 1-12. PMID: 37322158
- 12. Haiping Hao, Yang Tian, et al. "Chemoproteomic Mapping of Glycolytic Targetome in Cancer Cells." Research Square. 05 Oct, 2022.
- 13. Rachel Shvartsur, Galila Agam, et al. "Low-Dose Aspirin Augments the Anti-Inflammatory Effects of Low-Dose Lithium in Lipopolysaccharide-Treated Rats." Pharmaceutics. 2022 Apr 20;14(5):901. PMID: 35631487
- 14. Thuy T P Nguyen, Do-Young Kim, et al. "SREBP-1c impairs ULK1 sulfhydration-mediated autophagic flux to promote hepatic steatosis in high-fat-diet-fed mice." Mol Cell. 2021 Sep 16;81(18):3820-3832.e7. PMID: 34233158
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A8524 | Sodium Orthovanadate | PTP inhibitor | Membrane Transporter/Ion Channel|ATPase | 13721-39-6 | [O-][V](=O)([O-])[O-].[Na+].[Na+].[Na+] |
| B7843 | Sodium molybdate | Acid and phosphoprotein phosphatases inhibitor | 7631-95-0 | [O-][Mo](=O)([O-])=O.[Na+].[Na+] | |
| B7844 | Sodium tartrate | Acid phosphatases inhibitor | 868-18-8 | C(C(C(=O)[O-])O)(C(=O)[O-])O.[Na+].[Na+] | |
| B7845 | Imidazole | Alkaline phosphatases inhibitor | 288-32-4 | C1=CN=CN1 | |
| B7846 | sodium fluoride | Acid phosphatases inhibitor | 7681-49-4 | [F-].[Na+] | |
| Stored at -20°C, and stable for at least 12 months. When kept at 2-8°C, the product remains stable for 2 months. | |||||
