Phosphatase Inhibitor Cocktail 2 (100X in ddH2O)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Protein phosphorylation is an important covalent post-translational modification that can alter the structural conformation of a protein, which then regulates the function, location and specific binding of the target protein. Many cellular processes are regulated by the reversible phosphorylation of proteins and 30% of the proteins are likely to be phosphorylated at some point during their existence.
Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading and modifying proteins in the extracts. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.
Phosphatase Inhibitor Cocktail 2 inhibits tyrosine protein phosphatases, acid and alkaline phosphatases. This phosphatase inhibitor cocktail has been optimized and tested on cell extracts from various animal tissues.
This phosphatase inhibitor cocktail contains individual components, including Sodium orthovanadate, Sodium molybdate, Sodium tartrate, Imidazole and Sodium Fluoride. This phosphatase inhibitor cocktail is supplied as a ready-to-use solution in ddH2O.
Quality Control & DataSheet
- View current batch:
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Components and Storage
|Catalog No.||Product Name||Summary||Targets||CAS Number||Smiles|
|A8524||Sodium Orthovanadate||PTP inhibitor||Membrane Transporter/Ion Channel|ATPase||13721-39-6||[O-][V](=O)([O-])[O-].[Na+].[Na+].[Na+]|
|B7843||Sodium molybdate||Acid and phosphoprotein phosphatases inhibitor||7631-95-0||[O-][Mo](=O)([O-])=O.[Na+].[Na+]|
|B7844||Sodium tartrate||Acid phosphatases inhibitor||868-18-8||C(C(C(=O)[O-])O)(C(=O)[O-])O.[Na+].[Na+]|
|B7845||Imidazole||Alkaline phosphatases inhibitor||288-32-4||C1=CN=CN1|
|B7846||sodium fluoride||Acid phosphatases inhibitor||7681-49-4||[F-].[Na+]|
|Stored at -20°C, and stable for at least 12 months. When kept at 2-8°C, the product remains stable for 2 months.|