Phosphatase Inhibitor Cocktail 2 (100X in ddH2O)
Many cellular processes are regulated by reversible phosphorylation of proteins, and approximately 30% of proteins may become phosphorylated during their lifetime. Cellular crude extracts contain numerous endogenous enzymes, such as phosphatases and proteases, which can degrade and modify proteins present in the extract. Addition of inhibitors for these enzymes is the optimal approach for maximizing intact protein yield.
Phosphatase Inhibitor Cocktail 2 inhibits tyrosine protein phosphatases, acid phosphatases, and alkaline phosphatases. Phosphatase Inhibitor Cocktail 2 has been optimized and tested in cell extracts from multiple animal tissues. This Phosphatase Inhibitor Cocktail 2 contains Sodium orthovanadate, Sodium molybdate, Sodium tartrate, Imidazole, and Sodium Fluoride. It is supplied as a ready-to-use solution in ddH2O.
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Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A8524 | Sodium Orthovanadate | PTP inhibitor | Membrane Transporter/Ion Channel|ATPase | 13721-39-6 | [O-][V](=O)([O-])[O-].[Na+].[Na+].[Na+] |
| B7843 | Sodium molybdate | Acid and phosphoprotein phosphatases inhibitor | 7631-95-0 | [O-][Mo](=O)([O-])=O.[Na+].[Na+] | |
| B7844 | Sodium tartrate | Acid phosphatases inhibitor | 868-18-8 | C(C(C(=O)[O-])O)(C(=O)[O-])O.[Na+].[Na+] | |
| B7845 | Imidazole | Alkaline phosphatases inhibitor | 288-32-4 | C1=CN=CN1 | |
| B7846 | sodium fluoride | Acid phosphatases inhibitor | 7681-49-4 | [F-].[Na+] | |
| Stored at -20°C, and stable for at least 12 months. When kept at 2-8°C, the product remains stable for 2 months. | |||||
