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Phosphatase Inhibitor Cocktail (2 Tubes, 100X)

Catalog No.
K1015
Add to Compare
K1012+K1013
Grouped product items
SizePriceStock Qty
1ml A + 1ml B
$50.00
In stock
10x(1ml A + 1ml B)
$270.00
In stock
50x(1ml A + 1ml B)
$1,080.00
In stock
100x(1ml A + 1ml B)
$1,620.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

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Description

Protein phosphorylation is an important covalent posttranslational modification that changes the conformation of proteins and regulates the function, location and specific binding of target proteins. Many cellular processes are regulated by reversible phosphorylation of proteins, and 30% of proteins may be phosphorylated in their presence.

Due to the balance of endogenous protein production and degradation, its cell level is stable under stable environmental conditions. The crude cell extract contains many endogenous enzymes, such as phosphatase and protease, which degrade and alter the proteins in the extract. The best way to increase complete protein production is to add inhibitors of these enzymes.

More inhibitors mean better phosphorylation protection. We offer two tubes of inhibitors with different solution forms to help you better inhibit phosphatase.

Tube A (phosatase Inhibitor Cocktail 1, Catalog No. K1012) inhibits l-isozyme of alkaline Phosphatase and serine/threonine protein phosphatases, such as protein Phosphatase 1 and 2A (PP1 and PP2A). Tube A contains Cantharidin, Bromotetramisole and Microcystin LR, which are provided in the solution of DMSO.

Tube B (phosatase Inhibitor Cocktail 1, Catalog No. K1013) inhibits tyrosine protein Phosphatase, acid Phosphatase and alkaline Phosphatase. Tube B contains Sodium orthovanadate, Sodium molybdate, Sodium tartrate, Imidazole and Sodium Fluoride, with ddH2O box solution is provided.

Product Citation

Protocol

Thawed at room temperature, then diluted to solution samples (such as cell lysates or tissue extracts) at 1:100 (v/v) before the experiment, add A tube and B tube successively: add A tube, mix well; Add B tube, mix well (do not mix A tube and B tube and then add together).
WB，Co-IP，pull-down，IF，IHC，kinase assay and etc.

Quality Control

Quality Control & DataSheet

View current batch:

Related Biological Data

Phosphatase Inhibitor Cocktail (2 Tubes, 100X)

Related Biological Data

Phosphatase Inhibitor Cocktail (2 Tubes, 100X)
 

Components and Storage

Tube # Catalog No. Product Name Summary Targets CAS Number Smiles
A (100X in DMSO)
N1686 Cantharidin Protein phosphatase 1/2a inhibitor Natural Products 56-25-7 O=C([C@@]1(C([H])([H])[H])[C@]2(C([H])([H])[H])[C@@]3([H])O[C@]1([H])C([H])([H])C3([H])[H])OC2=O
B4750 (-)-p-Bromotetramisole Oxalate ALP inhibitor Others|Others 62284-79-1 BrC(C=C1)=CC=C1[C@H]2N=C3SCCN3C2.OC(C(O)=O)=O
B3698 Microcystin-LR Protein phosphatase PP1/PP2A inhibitor Chromatin/Epigenetics|Protein Ser/Thr Phosphatases 101043-37-2 CC1C(NC(=O)C(NC(=O)C(C(NC(=O)C(NC(=O)C(NC(=O)C(=C)N(C(=O)CCC(NC1=O)C(=O)O)C)C)CC(C)C)C(=O)O)C)CCCN=C(N)N)C=CC(=CC(C)C(CC2=CC=CC=C2)OC)C
B (100X in ddH2O)
A8524 Sodium Orthovanadate PTP inhibitor Membrane Transporter/Ion Channel|ATPase 13721-39-6 [O-][V](=O)([O-])[O-].[Na+].[Na+].[Na+]
B7843 Sodium molybdate Acid and phosphatase inhibitor 7631-95-0 [O-][Mo](=O)([O-])=O.[Na+].[Na+]
B7844 Sodium tartrate Acid phosphatase inhibitor 868-18-8 C(C(C(=O)[O-])O)(C(=O)[O-])O.[Na+].[Na+]
B7845 Imidazole Alkaline phosphatase inhibitor 288-32-4 C1=CN=CN1
B7846 sodium fluoride Acid phosphatase inhibitor/td> 7681-49-4 [F-].[Na+]
Store at -20°C for about 12 months. Store at 2-8°C for about 2 months.

Storage

Store at -20°C, stable storage for more than 12 months. When store at 2-8°C, the product will be stable for 2 months.