Phosphatase Inhibitor Cocktail (2 Tubes, 100X)

Protein phosphorylation, which dynamically regulates protein functions, localization, and interactions, mediates numerous cellular processes. Detecting and characterizing phosphorylation states requires protection from endogenous phosphatases that reverse phosphorylation during sample preparation. The Phosphatase Inhibitor Cocktail is composed of two distinct inhibitor formulations: Tube A, provided in DMSO, targets serine/threonine protein phosphatases (PP1, PP2A isoforms) and alkaline phosphatase isoenzymes through inhibitors such as Cantharidin, Bromotetramisole, and Microcystin LR; Tube B, in aqueous solution, inhibits tyrosine phosphatases, acid and alkaline phosphatases using Sodium orthovanadate, Sodium molybdate, Sodium tartrate, Imidazole, and Sodium Fluoride. This cocktail enables researchers to maintain phosphorylation integrity during cell lysis and sample processing for downstream biochemical assays, including immunoblotting, immunoprecipitation, kinase activity analyses, and mass spectrometry.

Protein phosphorylation is an important covalent posttranslational modification that changes the conformation of proteins and regulates the function, location and specific binding of target proteins. Many cellular processes are regulated by reversible phosphorylation of proteins, and 30% of proteins may be phosphorylated in their presence.

Due to the balance of endogenous protein production and degradation, its cell level is stable under stable environmental conditions. The crude cell extract contains many endogenous enzymes, such as phosphatase and protease, which degrade and alter the proteins in the extract. The best way to increase complete protein production is to add inhibitors of these enzymes.

More inhibitors mean better phosphorylation protection. We offer two tubes of inhibitors with different solution forms to help you better inhibit phosphatase.

Tube A (phosatase Inhibitor Cocktail 1, Catalog No. K1012) inhibits l-isozyme of alkaline Phosphatase and serine/threonine protein phosphatases, such as protein Phosphatase 1 and 2A (PP1 and PP2A). Tube A contains Cantharidin, Bromotetramisole and Microcystin LR, which are provided in the solution of DMSO.

Tube B (phosatase Inhibitor Cocktail 1, Catalog No. K1013) inhibits tyrosine protein Phosphatase, acid Phosphatase and alkaline Phosphatase. Tube B contains Sodium orthovanadate, Sodium molybdate, Sodium tartrate, Imidazole and Sodium Fluoride, with ddH2O box solution is provided.