Deacetylase Inhibitor Cocktail (100X in 70% DMSO)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Deacetylase Inhibitor Cocktail is a mixture of several chemicals with synergistic functions which is designed to maintain the acetylation state of molecules. Among these targets, dynamic changes of histone acetylation caused by HATs and HDACs are the most often occurring. Lysine acetylation is one of the most important post-translational modification which affects protein transcription, DNA replication and repair, cell cycle, and signal transduction pathways. The balance between acetyltransferases and deacetylases affects chromatin condensation, transcription factor activity and protein aggregation, these modification changes attracts significant interests from researchers. Such, Deacetylation Inhibition Cocktail is a useful tool for assays in these important pathways.
Four components of Deacetylase Inhibitor Cocktail: 40μM Trichostatin A, 1mM EX-527, 400 mM nicotinamide and 200mM sodium butyrate. The compounds are solubilized in 70% DMSO. Each has their own specific capacities. Trichostatin A can inhibit class I/II mammalian HDACs. EX-527 inhibits SIRT1 selectively with less activity on SIRT2 and SIRT3. Nicotinamide could act as an inhibitor of sirtuins (SIRT1-7, Class III HDAC). Sodium Butyrate acts against class I/II HDAC competitively.
Thaw on ice, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay and etc.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A8183 | Trichostatin A (TSA) | HDAC inhibitor | DNA Damage/DNA Repair|HDAC | 58880-19-6 | CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C |
| A4181 | EX 527 (SEN0014196) | SIRT1 inhibitor | Chromatin/Epigenetics|Sirtuin | 49843-98-3 | C1CC(C2=C(C1)C3=C(N2)C=CC(=C3)Cl)C(=O)N |
| N1651 | Nicotinamide | PARP-1 inhibitor | Nature Products | 98-92-0 | C1=CC(=CN=C1)C(=O)N |
| B1835 | Sodium butyrate | Histone deacetylase inhibitor | 156-54-7 | CCCC(=O)[O-].[Na+] | |
| Store at -20°C, and stable for at least 12 months. | |||||