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Deacetylase Inhibitor Cocktail (100X in 70% DMSO)

Catalog No.
K1017
maintain the acetylation state of proteins
Grouped product items
SizePriceStock Qty
1ml
$32.00
In stock
10x1ml
$160.00
In stock
50x1ml
$640.00
In stock
100x1ml
$960.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

Deacetylase Inhibitor Cocktail is a mixture of several chemicals with synergistic functions which is designed to maintain the acetylation state of molecules. Among these targets, dynamic changes of histone acetylation caused by HATs and HDACs are the most often occurring. Lysine acetylation is one of the most important post-translational modification which affects protein transcription, DNA replication and repair, cell cycle, and signal transduction pathways. The balance between acetyltransferases and deacetylases affects chromatin condensation, transcription factor activity and protein aggregation, these modification changes attracts significant interests from researchers. Such, Deacetylation Inhibition Cocktail is a useful tool for assays in these important pathways.

Four components of Deacetylase Inhibitor Cocktail: 40μM Trichostatin A, 1mM EX-527, 400 mM nicotinamide and 200mM sodium butyrate. The compounds are solubilized in 70% DMSO. Each has their own specific capacities. Trichostatin A can inhibit class I/II mammalian HDACs. EX-527 inhibits SIRT1 selectively with less activity on SIRT2 and SIRT3. Nicotinamide could act as an inhibitor of sirtuins (SIRT1-7, Class III HDAC). Sodium Butyrate acts against class I/II HDAC competitively.

Protocol

Thaw on ice, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.

Applications: WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay and etc.

Quality Control

Quality Control & MSDS

View current batch:
 

Components and Storage

Catalog No. Product Name Summary Targets CAS Number Smiles
A8183 Trichostatin A (TSA) HDAC inhibitor DNA Damage/DNA Repair|HDAC 58880-19-6 CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C
A4181 EX 527 (SEN0014196) SIRT1 inhibitor Chromatin/Epigenetics|Sirtuin 49843-98-3 C1CC(C2=C(C1)C3=C(N2)C=CC(=C3)Cl)C(=O)N
N1651 Nicotinamide PARP-1 inhibitor Nature Products 98-92-0 C1=CC(=CN=C1)C(=O)N
B1835 Sodium butyrate Histone deacetylase inhibitor 156-54-7 CCCC(=O)[O-].[Na+]
Store at -20°C, and stable for at least 12 months.

Storage

Store at -20°C, and stable for at least 12 months.