Deacetylase Inhibitor Cocktail (100X in 70% DMSO)
Deacetylase Inhibitor Cocktail (100× in 70% DMSO) is a reagent mixture designed to maintain protein acetylation states by targeting multiple classes of histone deacetylases (HDACs). It contains a combination of four compounds: Trichostatin A inhibiting class I/II HDACs; Sodium butyrate, functioning as a competitive inhibitor for the same HDAC classes; EX-527 specifically inhibiting SIRT1, with limited effects on SIRT2 and SIRT3; and Nicotinamide, acting as a broad inhibitor of Sirtuin enzymes. This cocktail is suited for cell biology and epigenetic studies where steady-state acetylation modifications are analyzed and offers researchers a useful approach to investigate chromatin remodeling, gene transcription regulation, DNA repair, signaling pathways, and cell-cycle dynamics.
- 1. Mark W. Pataky, Carrie J. Heppelmann, et al. "Aerobic and resistance exercise-regulated phosphoproteome and acetylproteome modifications in human skeletal muscle." Nat Commun. 2025 Jul 1;16(1):5700 PMID: 40595512
- 2. Johnson, Benjamin Stuart, et al. "Targeted degradation of mitochondrial aspartyl-tRNA synthetase (DARS2) modulates immune responses in experimental pneumonia." 2024, Doctor of Philosophy, Ohio State University, Biomedical Sciences.
- 3. Benjamin S Johnson, Daniela Farkas, et al. "Targeted degradation of extracellular mitochondrial aspartyl-tRNA synthetase modulates immune responses." Nat Commun. 2024 Jul 22;15(1):6172 PMID: 39039092
- 4. Abigail L D Tadenev, Courtney L Hatton, et al. "Lack of effect from genetic deletion of Hdac6 in a humanized mouse model of CMT2D." J Peripher Nerv Syst. 2024 Mar 29. PMID: 38551018
- 5. Zijiao Tang, Lu Liu, et al. "Combined inhibition of histone deacetylase and cytidine deaminase improves epigenetic potency of decitabine in colorectal adenocarcinomas." Clin Epigenetics. 2023 May 19;15(1):89. PMID: 37208732
- 6. Yan Yan, Xinming Wang, et al. "X-linked ubiquitin-specific peptidase 11 increases tauopathy vulnerability in women." Cell. 2022 Oct 13;185(21):3913-3930.e19. PMID: 36198316
- 7. Yunpeng Wang, Jiaoyu Mao, et al. "lncRNA HOTAIR mediates OGD/R‑induced cell injury and angiogenesis in a EZH2‑dependent manner." Exp Ther Med. 2022 Jan;23(1):99. PMID: 34976141
- 8. Gaojie Song, Jinbo Fang, et al. "Ad-apoptin inhibits glycolysis, migration and invasion in lung cancer cells targeting AMPK/mTOR signaling pathway." Exp Cell Res. 2021 Dec 15;409(2):112926. PMID: 34793774
- 9. Thuy T P Nguyen, Do-Young Kim, et al. "SREBP-1c impairs ULK1 sulfhydration-mediated autophagic flux to promote hepatic steatosis in high-fat-diet-fed mice." Mol Cell. 2021 Sep 16;81(18):3820-3832.e7. PMID: 34233158
Thaw on ice, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay and etc.
Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
A8183 | Trichostatin A (TSA) | HDAC inhibitor | DNA Damage/DNA Repair|HDAC | 58880-19-6 | CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C |
A4181 | EX 527 (SEN0014196) | SIRT1 inhibitor | Chromatin/Epigenetics|Sirtuin | 49843-98-3 | C1CC(C2=C(C1)C3=C(N2)C=CC(=C3)Cl)C(=O)N |
N1651 | Nicotinamide | PARP-1 inhibitor | Natural Products | 98-92-0 | C1=CC(=CN=C1)C(=O)N |
B1835 | Sodium butyrate | Histone deacetylase inhibitor | 156-54-7 | CCCC(=O)[O-].[Na+] | |
Store at -20°C, and stable for at least 12 months. |