Deacetylase Inhibitor Cocktail (100X in 70% DMSO)
The deacetylase inhibitor mixture (100×, dissolved in 70% DMSO) is a compound mixture designed to maintain protein acetylation status by targeting multiple classes of histone deacetylases (HDACs). It contains four compounds: Trichostatin A inhibits class I/II HDACs; sodium butyrate inhibits HDACs; EX-527 specifically inhibits SIRT1 with limited effects on SIRT2 and SIRT3; and nicotinamide, a broad inhibitor of deacetylases. This mixture is suitable for cell biology and epigenetics research and provides researchers with a useful tool to study chromatin remodelling, gene transcription regulation, DNA repair, signalling pathways, and cell cycle dynamics.
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Thaw on ice, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay and etc.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A8183 | Trichostatin A (TSA) | HDAC inhibitor | DNA Damage/DNA Repair|HDAC | 58880-19-6 | CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C |
| A4181 | EX 527 (SEN0014196) | SIRT1 inhibitor | Chromatin/Epigenetics|Sirtuin | 49843-98-3 | C1CC(C2=C(C1)C3=C(N2)C=CC(=C3)Cl)C(=O)N |
| N1651 | Nicotinamide | PARP-1 inhibitor | Natural Products | 98-92-0 | C1=CC(=CN=C1)C(=O)N |
| B1835 | Sodium butyrate | Histone deacetylase inhibitor | 156-54-7 | CCCC(=O)[O-].[Na+] | |
| Store at -20°C, and stable for at least 12 months. | |||||