Deacetylase Inhibitor Cocktail (100X in 70% DMSO)
Deacetylase Inhibitor Cocktail is composed of several compounds that act synergistically to maintain the acetylation status of molecules. The balance between acetyltransferase and deacetylase activities impacts chromatin compaction, transcription factor activity, and protein aggregation, and the variations of these modifications have garnered considerable interest from researchers.
Our product, Deacetylase Inhibitor Cocktail, is a valuable tool for investigating these changes.The Deacetylation Inhibition Cocktail includes four active components, 40 µM Trichostatin A, 1 mM EX-527, 400 mM nicotinamide, and 200 mM sodium butyrate. These compounds are dissolved in 70% DMSO, each with distinct specific actions. Trichostatin A inhibits class I/II HDACs. EX-527 selectively inhibits SIRT1, with weaker activity against SIRT2 and SIRT3. Nicotinamide acts as an inhibitor of sirtuins (SIRT1-7,class III HDACs). Sodium butyrate competitively inhibits class I/II HDACs.
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Thaw on ice, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay and etc.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A8183 | Trichostatin A (TSA) | HDAC inhibitor | DNA Damage/DNA Repair|HDAC | 58880-19-6 | CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C |
| A4181 | EX 527 (SEN0014196) | SIRT1 inhibitor | Chromatin/Epigenetics|Sirtuin | 49843-98-3 | C1CC(C2=C(C1)C3=C(N2)C=CC(=C3)Cl)C(=O)N |
| N1651 | Nicotinamide | PARP-1 inhibitor | Nature Products | 98-92-0 | C1=CC(=CN=C1)C(=O)N |
| B1835 | Sodium butyrate | Histone deacetylase inhibitor | 156-54-7 | CCCC(=O)[O-].[Na+] | |
| Store at -20°C, and stable for at least 12 months. | |||||