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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Sodium molybdate is an acid phosphatase inhibitor. The osteoclastic acid phosphatase isoenzyme, secreted by osteoclasts, is a member of a widely-distributed class of iron-containing proteins with acid phosphatase activity. Elevated plasma isoenzyme levels are associated with increased bone turnover in metabolic disease. By inhibiting this class of acid phosphatases, sodium molybdate is capable of abolishing bone resorption. In addition, sodium molybdate has been shown to stabilize the nonactivated glucocorticoid-receptor complex.
References:
1. Zaidi M, Moonga B, Moss DW, et al. Inhibition of osteoclastic acid phosphatase abolishes bone resorption. Biochemical and Biophysical Research Communications, 1989, 159(1): 68-71.
2. Denis M, Wikström AC, Gustafsson JA. The molybdate-stabilized nonactivated glucocorticoid receptor contains a dimer of Mr 90,000 non-hormone-binding protein. Journal of Biological Chemistry, 1987, 262(24): 11803-11806.
Cell lines
Rat osteoclasts
Reaction Conditions
1 ~ 100 μM sodium molybdate
Applications
Addition of the phosphatase inhibitor sodium molybdate to the bone-osteoclast cultures at a concentration of 100 μM abolished bone resorption and reduced tartrate-resistant acid phosphatase activity by about 40%.
Note
The technical data provided above is for reference only.