Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)
Catalog No.
K1010
Protease Inhibitor Cocktail similar to Sigma P8849
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Protease inhibitor cocktails (similar to Sigma P8849) are commonly used in protein extraction to prevent protein breakdown by natural cellular proteases. These enzymes (including cysteine, serine, acid proteases, and aminopeptidases) are activated when cells or tissues are disrupted and can damage target proteins. This ready-to-use 100X DMSO solution uses a mix of inhibitors (AEBSF, Bestatin, E-64, Leupeptin, Pepstatin A) to block such proteases, preserving proteins’ natural structure and properties. Being EDTA-free, it works well for downstream steps sensitive to divalent cations, like phosphorylation analysis or enzyme assays.
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Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A2573 | AEBSF.HCl | Serine protease inhibitor | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl |
| A2575 | Bestatin | Aminopeptidase inhibitor | Proteases|Aminopeptidase | 58970-76-6 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O |
| A2576 | E-64 | Cysteine protease inhibitor,irriversible | Proteases|Cathepsin | 66701-25-5 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O |
| A2571 | Pepstatin A | Aspartic protease inhibitor | Proteases|Other Proteases | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O |
| B4790 | Phosphoramidon Disodium Salt | metalloproteinase inhibitor | 164204-38-0 | CC(C[C@@](NP(O)(O[C@@]1([H])[C@@](O)([H])[C@@](O)([H])[C@](O)([H])[C@](O1)([H])C)=O)([H])/C([O-])=N/[C@@](C([O-])=O)([H])CC2=CNC3=CC=CC=C23)C.[Na+].[Na+] | |
| Stored at -20°C, and stable for at least 12 months. | |||||
Stored at -20°C, and stable for at least 12 months.
