Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)
Cellular crude extracts contain numerous endogenous enzymes, such as phosphatases and proteases, capable of degrading proteins in the extracts. The most effective strategy to enhance the yield of intact proteins is through the addition of inhibitors targeting these known endogenous enzymes.The Protease Inhibitor Cocktail is intended for the purification of His-tagged proteins to improve protein stability. This cocktail functions by inhibiting proteases that degrade either phosphorylated or non-phosphorylated protein substrates.
This Protease Inhibitor Cocktail contains individual components including AEBSF, Bestatin, E-64, Phosphoramidon, and Pepstatin A, offering broad-spectrum specific inhibition against cysteine, serine, aspartic, thermolysin-like proteases, and aminopeptidases. It has been optimized and validated specifically for use with His-tagged proteins. The cocktail is dissolved in DMSO and supplied as a ready-to-use solution.
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Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A2573 | AEBSF.HCl | Serine protease inhibitor | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl |
| A2575 | Bestatin | Aminopeptidase inhibitor | Proteases|Aminopeptidase | 58970-76-6 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O |
| A2576 | E-64 | Cysteine protease inhibitor,irriversible | Proteases|Cathepsin | 66701-25-5 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O |
| A2571 | Pepstatin A | Aspartic protease inhibitor | Proteases|Other Proteases | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O |
| B4790 | Phosphoramidon Disodium Salt | metalloproteinase inhibitor | 164204-38-0 | CC(C[C@@](NP(O)(O[C@@]1([H])[C@@](O)([H])[C@@](O)([H])[C@](O)([H])[C@](O1)([H])C)=O)([H])/C([O-])=N/[C@@](C([O-])=O)([H])CC2=CNC3=CC=CC=C23)C.[Na+].[Na+] | |
| Stored at -20°C, and stable for at least 12 months. | |||||
