| Protease Inhibitor Cocktail(EDTA-Free,100X in DMSO)
Equivalent to Sigma P8340, for use with cell and tissue extracts to increase protein stability |
Sample solution is provided at 25 µL, 10mM.
- 1. Lu Xianyuan,Zou Wei, et al. "Anti-renal fibrosis effect of asperulosidic acid via TGF-β1/smad2/smad3 and NF-κB signaling pathways in a rat model of unilateral ureteral obstruction." Phytomedicine Available online 5 September 2018.
- 2. Michele Renae Plewes. "The Effects of in Vitro Fish Oil and Dietary Fish Meal Supplementation on Microdomains Within Bovine Luteal Cells." University of Northern Colorado.2018.
- 3. Shi G, Zhang H, et al. "Epigenetic silencing of sonic hedgehog elicits antitumor immune response and suppresses tumor growth by inhibiting the hedgehog signaling pathway in metastatic spine tumors in Sprague-Dawley rats." J Cell Biochem. 2018 Nov;119(11):9591-9603. PMID:30191602
- 4. Gao X, Bao S, et al. "Fructose-1,6-bisphosphate aldolase of Mycoplasma bovis is a plasminogen-binding adhesin." Microb Pathog. 2018 Aug 22;124:230-237. PMID:30142464
- 5. M.R. Plewes, P.D. Burns. "Effect of fish oil on agonist-induced receptor internalization of the prostaglandin F 2α receptor and cell signaling in bovine luteal cells in vitro." Domestic Animal Endocrinology. 2017 December 12.
- 6. Rana PS, Mudrak NJ, et al. "Phase separation in necrotic cells." Biochem Biophys Res Commun. 2017 Oct 21;492(3):300-303. PMID:28859980
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A2573 | AEBSF.HCl | Serine protease inhibitor | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl |
| A2574 | Aprotinin | Inhibitor of bovine pancreatic trypsin | Proteases|Serine Protease | 9087-70-1 | |
| A2575 | Bestatin | Aminopeptidase inhibitor | Proteases|Aminopeptidase | 58970-76-6 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O |
| A2576 | E-64 | Cysteine protease inhibitor,irreversible | Proteases|Cathepsin | 66701-25-5 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O |
| A2570 | Leupeptin | Inhibitor of serine and cysteine proteases | Proteases|Serine Protease | 103476-89-7 | O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] |
| A2571 | Pepstatin A | Aspartic proteinases inhibitor | Proteases|Other Proteases | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O |
| Stored at -20°C, and stable for at least 12 months. | |||||
Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.
Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates.
This protease inhibitor cocktail contains individual components, including AEBSF, Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A with a broad specificity for cysteine, serine, acid proteases, and aminopeptidases. This protease inhibitor cocktail has been optimized and tested for mammalian cells and tissue extracts. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.
 |
Accurate Ingredients: Clearance of components and concentrations. Higher efficiency: Quick achievement, quality assurance. Lower price: Our price is less than any of competitor. |