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Phosphatase Inhibitor Cocktail 1 (100X in DMSO)

Catalog No.
K1012
Phosphatase Inhibitor Cocktail
Grouped product items
SizePriceStock Qty
1ml
$50.00
In stock
10x1ml
$315.00
In stock
50x1ml
$1,330.00
In stock
100x1ml
$1,990.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

Protein phosphorylation, which dynamically regulates protein functions, localization, and interactions, mediates numerous cellular processes. Detecting and characterizing phosphorylation states requires protection from endogenous phosphatases that reverse phosphorylation during sample preparation. Phosphatase Inhibitor Cocktail 1 provides a formulated mixture of cantharidin, bromotetramisole, and microcystin LR, dissolved in DMSO at a 100X concentration. This inhibitor cocktail targets alkaline phosphatases and serine/threonine phosphatases, preserving phosphorylation states during protein extraction from various animal tissues and cultured cells, supporting accurate characterization in phosphoproteomic analyses and downstream biochemical assays.

Product Citation

Quality Control

Quality Control & DataSheet

View current batch:

Related Biological Data

Phosphatase Inhibitor Cocktail 1 (100X in DMSO)
 

Protocol

Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components and Storage

Catalog No. Product Name Summary Targets CAS Number Smiles
N1686 Cantharidin Inhibitor of protein phosphatases 1/2A Natural Products 56-25-7 O=C([C@@]1(C([H])([H])[H])[C@]2(C([H])([H])[H])[C@@]3([H])O[C@]1([H])C([H])([H])C3([H])[H])OC2=O
B4750 (-)-p-Bromotetramisole Oxalate ALP inhibitor, potent and non-specific Others|Others 62284-79-1 BrC(C=C1)=CC=C1[C@H]2N=C3SCCN3C2.OC(C(O)=O)=O
B3698 Microcystin-LR Inhibitor of protein phosphatase PP1/PP2A,potent and selective Chromatin/Epigenetics|Protein Ser/Thr Phosphatases 101043-37-2 CC1C(NC(=O)C(NC(=O)C(C(NC(=O)C(NC(=O)C(NC(=O)C(=C)N(C(=O)CCC(NC1=O)C(=O)O)C)C)CC(C)C)C(=O)O)C)CCCN=C(N)N)C=CC(=CC(C)C(CC2=CC=CC=C2)OC)C
Stored at -20°C, and stable for at least 12 months. When kept at 2-8°C, the product remains stable for 2 months.

Storage

Stored at -20°C, and stable for at least 12 months. When kept at 2-8°C, the product remains stable for 2 months.