Phosphatase Inhibitor Cocktail 1 (100X in DMSO)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Protein phosphorylation is an important covalent post-translational modification that can alter the structural conformation of a protein, which then regulates the function, location and specific binding of the target protein. Many cellular processes are regulated by the reversible phosphorylation of proteins and 30% of the proteins are likely to be phosphorylated at some point during their existence.
Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading and modifying proteins in the extracts. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.
Phosphatase Inhibitor Cocktail 1 inhibits L-isozymes of alkaline phosphatases and serine/threonine protein phosphatases such as protein phosphatases 1 and 2A (PP1 and PP2A). This phosphatase inhibitor cocktail has been optimized and tested on cell extracts from various animal tissues.
This phosphatase inhibitor cocktail contains individual components, including Cantharidin, Bromotetramisole and Microcystin LR. This phosphatase inhibitor cocktail is supplied as a ready-to-use solution in DMSO.
Quality Control & DataSheet
- View current batch:
Related Biological Data
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
- 1. Yu ZQ, Yi HY, et al. "Ginsenoside Rg1 Suppresses Type 2 PRRSV Infection via NF-κB Signaling Pathway In Vitro, and Provides Partial Protection against HP-PRRSV in Piglet." Viruses. 2019 Nov 10;11(11). pii: E1045. PMID:31717616
- 2. Losier TT, Akuma M, et al. "AMPK Promotes Xenophagy through Priming of Autophagic Kinases upon Detection of Bacterial Outer Membrane Vesicles." Cell Rep. 2019 Feb 19;26(8):2150-2165.e5. PMID:30784596
Components and Storage
|Catalog No.||Product Name||Summary||Targets||CAS Number||Smiles|
|N1686||Cantharidin||Inhibitor of protein phosphatases 1/2A||Natural Products||56-25-7||O=C([[email protected]@]1(C([H])([H])[H])[[email protected]]2(C([H])([H])[H])[[email protected]@]3([H])O[[email protected]]1([H])C([H])([H])C3([H])[H])OC2=O|
|B4750||(-)-p-Bromotetramisole Oxalate||ALP inhibitor, potent and non-specific||Others|Others||62284-79-1||BrC(C=C1)=CC=C1[[email protected]]2N=C3SCCN3C2.OC(C(O)=O)=O|
|B3698||Microcystin-LR||Inhibitor of protein phosphatase PP1/PP2A,potent and selective||Chromatin/Epigenetics|Protein Ser/Thr Phosphatases||101043-37-2||CC1C(NC(=O)C(NC(=O)C(C(NC(=O)C(NC(=O)C(NC(=O)C(=C)N(C(=O)CCC(NC1=O)C(=O)O)C)C)CC(C)C)C(=O)O)C)CCCN=C(N)N)C=CC(=CC(C)C(CC2=CC=CC=C2)OC)C|
|Stored at -20°C, and stable for at least 12 months. When kept at 2-8°C, the product remains stable for 2 months.|