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Phosphatase Inhibitor Cocktail 3 (100X in DMSO)

Catalog No.
K1014
Phosphatase Inhibitor Cocktail
Grouped product items
SizePriceStock Qty
1ml
$55.00
In stock
10x1ml
$395.00
In stock
50x1ml
$1,840.00
In stock
100x1ml
$2,765.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

Protein phosphorylation, which dynamically regulates protein functions, localization, and interactions, mediates numerous cellular processes. Detecting and characterizing phosphorylation states requires protection from endogenous phosphatases that reverse phosphorylation during sample preparation.  The Phosphatase Inhibitor Cocktail 3 contains a potent combination of inhibitors: Cantharidin, Bromotetramisole, and Calyculin A, dissolved in DMSO as a concentrated ready-to-use solution. This inhibitor cocktail targets alkaline phosphatases and serine/threonine-specific phosphatases, notably protein phosphatases PP1 and PP2A, and is routinely used during protein extraction procedures from various animal tissues and cultured cells to preserve phosphorylation status for subsequent phosphoprotein analysis.

Product Citation

Quality Control

Quality Control & DataSheet

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Related Biological Data

Phosphatase Inhibitor Cocktail 3 (100X in DMSO)
 

Protocol

Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components and Storage

Catalog No. Product Name Summary Targets CAS Number Smiles
N1686 Cantharidin Protein phosphatase 1/2a inhibitor Natural Products 56-25-7 O=C([C@@]1(C([H])([H])[H])[C@]2(C([H])([H])[H])[C@@]3([H])O[C@]1([H])C([H])([H])C3([H])[H])OC2=O
B4750 (-)-p-Bromotetramisole Oxalate ALP inhibitor Others|Others 62284-79-1 BrC(C=C1)=CC=C1[C@H]2N=C3SCCN3C2.OC(C(O)=O)=O
A4533 Calyculin A Protein phosphatase inhibitor Chromatin/Epigenetics|Protein Ser/Thr Phosphatases 101932-71-2 CC1C(CC2(C(C(C(O2)C(CC(C(C)C(C(C)C=C(C)C(=CC=CC(=CC#N)C)C)O)O)OC)OP(=O)(O)O)(C)C)OC1CC=CC3=COC(=N3)C(C)CCNC(=O)C(C(C(COC)N(C)C)O)O)O
Store at -20°C, stable storage for more than 12 months. When store at 2-8°C, the product will be stable for 2 months.

Storage

Store at -20°C, stable storage for more than 12 months. When store at 2-8°C, the product will be stable for 2 months.