Phosphatase Inhibitor Cocktail 3 (100X in DMSO)
Protein phosphorylation, which dynamically regulates protein functions, localization, and interactions, mediates numerous cellular processes. Detecting and characterizing phosphorylation states requires protection from endogenous phosphatases that reverse phosphorylation during sample preparation. The Phosphatase Inhibitor Cocktail 3 contains a potent combination of inhibitors: Cantharidin, Bromotetramisole, and Calyculin A, dissolved in DMSO as a concentrated ready-to-use solution. This inhibitor cocktail targets alkaline phosphatases and serine/threonine-specific phosphatases, notably protein phosphatases PP1 and PP2A, and is routinely used during protein extraction procedures from various animal tissues and cultured cells to preserve phosphorylation status for subsequent phosphoprotein analysis.
- 1. Damián Gatica, Reham M. Alsaadi, et al. "The ER-phagy receptor FAM134B is targeted by Salmonella Typhimurium to promote infection." Nat Commun. 2025 Mar 25;16(1):2923. PMID: 40133256
- 2. Yun Li, Xiangqian Zhang, et al. "RNF166 promotes colorectal cancer progression by recognizing and destabilizing poly-ADP-ribosylated angiomotins." Cell Death Dis. 2024 Mar 13;15(3):211. PMID: 38480683
- 3. Yang M, Mariano J, et al. "SARS-CoV-2 papain-like protease (PLpro) plays multiple roles in regulating cellular proteins in the endoplasmic reticulum." Journal of Biological Chemistry, 2023: 105346. PMID: 37838170
- 4. Thuy T P Nguyen, Do-Young Kim, et al. "SREBP-1c impairs ULK1 sulfhydration-mediated autophagic flux to promote hepatic steatosis in high-fat-diet-fed mice." Mol Cell. 2021 Sep 16;81(18):3820-3832.e7. PMID: 34233158
- 5. Alpsoy A, Dykhuizen EC. "Glioma tumor suppressor candidate region gene 1 (GLTSCR1) and its paralog GLTSCR1-like form SWI/SNF chromatin remodeling subcomplexes." J Biol Chem. 2018 Mar 16;293(11):3892-3903. PMID: 29374058
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| N1686 | Cantharidin | Protein phosphatase 1/2a inhibitor | Natural Products | 56-25-7 | O=C([C@@]1(C([H])([H])[H])[C@]2(C([H])([H])[H])[C@@]3([H])O[C@]1([H])C([H])([H])C3([H])[H])OC2=O |
| B4750 | (-)-p-Bromotetramisole Oxalate | ALP inhibitor | Others|Others | 62284-79-1 | BrC(C=C1)=CC=C1[C@H]2N=C3SCCN3C2.OC(C(O)=O)=O |
| A4533 | Calyculin A | Protein phosphatase inhibitor | Chromatin/Epigenetics|Protein Ser/Thr Phosphatases | 101932-71-2 | CC1C(CC2(C(C(C(O2)C(CC(C(C)C(C(C)C=C(C)C(=CC=CC(=CC#N)C)C)O)O)OC)OP(=O)(O)O)(C)C)OC1CC=CC3=COC(=N3)C(C)CCNC(=O)C(C(C(COC)N(C)C)O)O)O |
| Store at -20°C, stable storage for more than 12 months. When store at 2-8°C, the product will be stable for 2 months. | |||||
