Phosphatase Inhibitor Cocktail 3 (100X in DMSO)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Quality Control & DataSheet
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Related Biological Data
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Components and Storage
|Catalog No.||Product Name||Summary||Targets||CAS Number||Smiles|
|N1686||Cantharidin||Protein phosphatase 1/2a inhibitor||Natural Products||56-25-7||O=C([[email protected]@]1(C([H])([H])[H])[[email protected]]2(C([H])([H])[H])[[email protected]@]3([H])O[[email protected]]1([H])C([H])([H])C3([H])[H])OC2=O|
|B4750||(-)-p-Bromotetramisole Oxalate||ALP inhibitor||Others|Others||62284-79-1||BrC(C=C1)=CC=C1[[email protected]]2N=C3SCCN3C2.OC(C(O)=O)=O|
|A4533||Calyculin A||Protein phosphatase inhibitor||Chromatin/Epigenetics|Protein Ser/Thr Phosphatases||101932-71-2||CC1C(CC2(C(C(C(O2)C(CC(C(C)C(C(C)C=C(C)C(=CC=CC(=CC#N)C)C)O)O)OC)OP(=O)(O)O)(C)C)OC1CC=CC3=COC(=N3)C(C)CCNC(=O)C(C(C(COC)N(C)C)O)O)O|
|Store at -20°C, stable storage for more than 12 months. When store at 2-8°C, the product will be stable for 2 months.|
Protein phosphorylation is an important covalent post-translational modification that can alter the structural conformation of a protein, which then regulates the function, location and specific binding of the target protein. Many cellular processes are regulated by the reversible phosphorylation of proteins and 30% of the proteins are likely to be phosphorylated at some point during their existence.
Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading and modifying proteins in the extracts. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.
Phosphatase Inhibitor Cocktail 3 inhibits L-isozymes of alkaline phosphatases and serine/threonine protein phosphatases such as protein phosphatases 1 and 2A (PP1 and PP2A). This phosphatase inhibitor cocktail has been optimized and tested on cell extracts from various animal tissues. Phosphatase Inhibitor Cocktail 3 can replace Phosphatase Inhibitor Cocktail 1, and the activity of the two cocktails is equivalent.
This phosphatase inhibitor cocktail contains individual components, including Cantharidin, Bromotetramisole and Calyculin A. This phosphatase inhibitor cocktail is supplied as a ready-to-use solution in DMSO.