Phosphatase Inhibitor Cocktail 3 (100X in DMSO)
Protein phosphorylation, which dynamically regulates protein functions, localization, and interactions, mediates numerous cellular processes. Detecting and characterizing phosphorylation states requires protection from endogenous phosphatases that reverse phosphorylation during sample preparation. The Phosphatase Inhibitor Cocktail 3 contains a potent combination of inhibitors: Cantharidin, Bromotetramisole, and Calyculin A, dissolved in DMSO as a concentrated ready-to-use solution. This inhibitor cocktail targets alkaline phosphatases and serine/threonine-specific phosphatases, notably protein phosphatases PP1 and PP2A, and is routinely used during protein extraction procedures from various animal tissues and cultured cells to preserve phosphorylation status for subsequent phosphoprotein analysis.
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Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
N1686 | Cantharidin | Protein phosphatase 1/2a inhibitor | Natural Products | 56-25-7 | O=C([C@@]1(C([H])([H])[H])[C@]2(C([H])([H])[H])[C@@]3([H])O[C@]1([H])C([H])([H])C3([H])[H])OC2=O |
B4750 | (-)-p-Bromotetramisole Oxalate | ALP inhibitor | Others|Others | 62284-79-1 | BrC(C=C1)=CC=C1[C@H]2N=C3SCCN3C2.OC(C(O)=O)=O |
A4533 | Calyculin A | Protein phosphatase inhibitor | Chromatin/Epigenetics|Protein Ser/Thr Phosphatases | 101932-71-2 | CC1C(CC2(C(C(C(O2)C(CC(C(C)C(C(C)C=C(C)C(=CC=CC(=CC#N)C)C)O)O)OC)OP(=O)(O)O)(C)C)OC1CC=CC3=COC(=N3)C(C)CCNC(=O)C(C(C(COC)N(C)C)O)O)O |
Store at -20°C, stable storage for more than 12 months. When store at 2-8°C, the product will be stable for 2 months. |