Phosphatase Inhibitor Cocktail 3 (100X in DMSO)

Protein phosphorylation is an important covalent post-translational modification that can alter the structural conformation of a protein, which then regulates the function, location and specific binding of the target protein. Many cellular processes are regulated by the reversible phosphorylation of proteins and 30% of the proteins are likely to be phosphorylated at some point during their existence.

Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading and modifying proteins in the extracts. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Phosphatase Inhibitor Cocktail 3 inhibits L-isozymes of alkaline phosphatases and serine/threonine protein phosphatases such as protein phosphatases 1 and 2A (PP1 and PP2A). This phosphatase inhibitor cocktail has been optimized and tested on cell extracts from various animal tissues. Phosphatase Inhibitor Cocktail 3 can replace Phosphatase Inhibitor Cocktail 1, and the activity of the two cocktails is equivalent.

This phosphatase inhibitor cocktail contains individual components, including Cantharidin, Bromotetramisole and Calyculin A. This phosphatase inhibitor cocktail is supplied as a ready-to-use solution in DMSO.