5X Protein Loading Buffer (Reducing)

5X Protein Loading Buffer (Reducing) is an improved 5-fold concentrated protein loading buffer. Its main components include SDS, a sulfhydryl reducing agent, bromophenol blue, and buffer salts. SDS binds to proteins, imparting a large net negative charge to the protein-SDS complex, thereby masking the intrinsic charge differences among various proteins. SDS also disrupts intra- and inter-molecular hydrogen bonds, destroying the secondary and tertiary structures of protein molecules. The sulfhydryl reducing agent breaks disulfide bonds between cysteine residues, disrupting the quaternary structure of proteins and eliminating structural differences between proteins, so that the electrophoresis migration rate depends only on molecular weight. Bromophenol blue serves as a tracking dye during electrophoresis, indicating approximately when the run should be terminated.

5X Protein Loading Buffer (Reducing) is suitable for loading protein samples in conventional SDS-PAGE.