RIPA Lysis Buffer (Medium)

RIPA Lysis Buffer (Medium) is widely applicable to reporter gene assays, protein kinase assays, immunoassays, and protein purification. Its core components are 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP?40, 0.5% sodium deoxycholate, and 0.1% SDS, and it contains common protease and phosphatase inhibitors (sodium orthovanadate, sodium fluoride, EDTA, and leupeptin) to effectively prevent protein degradation. This lysis buffer does not contain a full spectrum of protease and phosphatase inhibitors; for optimal protein protection, please additionally add a comprehensive protease and phosphatase inhibitor cocktail.

This product is suitable for protein extraction from animal cells and tissues. The resulting protein samples can be used in various routine immunological assays, such as Western blotting (WB), immunoprecipitation (IP), and enzyme?linked immunosorbent assays (ELISA).

For 100 mL of this product, 150–250 μL of lysis buffer is required per well in a 6?well plate, allowing one bottle to be used for approximately 400–666 wells (samples). For tissue samples, 150–250 μL of lysis buffer is required per 20 mg of tissue, so one bottle can be used for approximately 400–666 extractions. The exact volume can be adjusted according to sample density and lysis efficiency.