Immunoprecipitation Kit (Protein G Agarose Gel)

Immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) is a common technique used to study proteins or protein-protein interactions (PPIs) by using specific antibodies and antibody-binding mediators (e.g., Protein A/ G agarose gels, etc.), or directly use a medium conjugated to a specific antibody (such as an agarose gel or magnetic beads) to separate the protein of interest from complex samples, which can then be used for Western blot detection or mass spectrometry analysis.

Protein G is an immunoglobulin-binding protein expressed by Streptococcal bacteria type C or G, which specifically binds to mammalian immunoglobulin (Ig). Fc zone binding. Protein G can bind to human IgG1, IgG2, IgG3, IgG4, mouse IgG1, IgG2a, IgG2b, IgG3, rat IgG1, IgG2a, IgG2b, IgG2c, rabbit, goat polyclonal antibodies, etc., and each Protein G molecule can bind 3 IgG molecules. The binding capacity of common immunoglobulin subclasses and the total binding capacity of different species are shown in the table below (Table 1).

This kit contains Protein A agarose gel and other necessary reagents for immunoprecipitation, and with the specific antibodies provided by the user, it makes immunoprecipitation (IP, also known as pull-down) or co-immunoprecipitation (Co-IP) experiments simpler, more convenient and efficient, and the immunoprecipitation products can be used for the detection of the target protein or its protein complexes and other experiments. (See Table 2 for specific product parameters)

Table 1 Affinity data for Protein G for different sources and subtypes of IgG. ++++ = Strong Binding; ++~+++ = Medium Binding; + = Weak Binding; +/- = Weak or No Binding; - = No Binding.

Table 2 The main related indicators of Protein G Agarose Gel.