NP-40 Lysis Buffer

NP-40 Lysis Buffer is a relatively mild cell and tissue lysis buffer used to prepare protein samples by lysing cells or tissue samples under non-denaturing conditions. The main components of this product include 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, as well as various inhibitors such as sodium pyrophosphate, β-glycerophosphate, sodium orthovanadate, sodium fluoride, EDTA, and leupeptin. These effectively prevent protein degradation and maintain native protein-protein interactions. Protein samples obtained using NP-40 Lysis Buffer can be used for routine PAGE, Western Blotting (WB), Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Enzyme-Linked Immunosorbent Assay (ELISA). This product can be used for animal and plant cell or tissue samples, as well as for fungal or bacterial samples.