Active Protein Extraction Reagent (Insect)

Active Protein Extraction Reagent (Insect) is a gentle, easy-to-use cell lysis reagent consisting primarily of a native detergent (Tris, pH 8.0). This product is mainly used for the extraction of insect active proteins from cytoplasmic proteins, nuclear proteins and membrane proteins of insect cells (such as Sf 9 and Sf 21) or insect tissues.
The extraction effect of this product for cytoplasmic proteins is better than that of nucleus and cell membrane proteins, and although it also has a good extraction effect on membrane proteins, the extraction efficiency of some insoluble proteins is relatively low. If you wish to remove the detergent from the protein solution extracted using this product, you can do so by dialysis or ultrafiltration.
The proteins extracted from this product typically maintain their original structure and biological activity very well. They can be used for various biochemical and molecular biology applications, and are compatible with downstream applications, including the separation and purification of tagged proteins such as His, GST, HA, Flag, Myc, and V5. They are also suitable for routine biochemical analyses such as Western blotting, immunoprecipitation (IP)/co-immunoprecipitation (Co-IP), and ELISA. Additionally, they can be used for reporter gene enzyme activity assays, including luciferase, β-galactosidase, alkaline phosphatase, and CAT. Furthermore, they are applicable for detecting protein kinase activity, such as PKA, PKC, and tyrosine kinase.。
This product is compatible with Thermo'sI-The PERTM series is very similar, and the use effect and use are very similar, and many times you can choose to replace each other.