Active Protein Extraction Reagent (Mammal)

Active Protein Extraction Reagent (Mammal) is a gentle, easy-to-use cell lysis reagent consisting primarily of a native detergent (Tris, pH 8.0). This product is mainly used for the extraction of active proteins from cytoplasmic proteins, nuclear proteins and membrane proteins of mammalian cells or tissues. If you wish to remove the detergent from the protein solution extracted using this product, you can do so by dialysis or ultrafiltration.
The proteins extracted by this product can usually maintain their original structures and biological activities well and can be used for a variety of biochemical and molecular biological purposes. Meanwhile, they are compatible with downstream applications, including the separation and purification of tagged proteins, such as His, GST, HA, Flag, Myc, V5 and other tagged proteins; conventional biochemical analyses, such as Western blot, IP/Co-IP, ELISA, etc.; the detection of reporter gene enzyme activities, such as luciferase, β-gal, alkaline phosphatase, CAT, etc.; and the detection of protein kinase activities, such as PKA, PKC and tyrosine kinase, etc.
This product is very similar to Thermo's M-PERTM series and Sigma's CelLytic™ series. Their application effects and uses are also quite close, and they can often be substituted for each other as options.