Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)
Crude cell extracts contain a variety of endogenous enzymes capable of degrading proteins, such as phosphatases and proteases. The optimal method to enhance the yield of intact proteins is to supplement with inhibitors targeting these known enzymes.This Protease Inhibitor Cocktail is used in plant cell and tissue extracts to improve protein stability. The cocktail inhibits proteases that degrade both non-phosphorylated and phosphorylated protein substrates.
The Protease Inhibitor Cocktail contains individual components including AEBSF, 1,10-Phenanthroline, Bestatin, E-64, Leupeptin, and Pepstatin A, providing broad specificity against cysteine, serine, aspartic, metalloproteases, and aminopeptidases. This protease inhibitor cocktail has been optimized and tested specifically for plant cell and tissue extracts. Provided as a ready-to-use solution, this protease inhibitor cocktail is supplied in DMSO as the solvent.
- 1. Chengli Fang，Kun Huang, et al. "Transcription elongation of the plant RNA polymerase IV is prone to backtracking." Sci Adv. 2024 Aug 23; 10(34): eadq3087. PMID: 39178250
- 2. Tianyi Xu, Haijing Fu, et al. "Far-infrared radiation alleviates steatohepatitis and fibrosis in metabolic dysfunction-associated fatty liver disease." Sci Rep. 2024 Aug 20;14(1):19292 PMID: 39164313
- 3. Lidong Wang, Yonglian Huang, et al. "Deltex3 inhibits Epithelial Mesenchymal Transition in Papillary Thyroid Carcinoma via promoting ubiquitination of XRCC5 to regulate the AKT signal pathway." J Cancer. 2021 Jan 1;12(3):860-873. PMID: 33403043
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as plant cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A2573 | AEBSF.HCl | Serine protease inhibitor | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl |
| A2575 | Bestatin | Aminopeptidase inhibitor | Proteases|Aminopeptidase | 58970-76-6 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O |
| A2576 | E-64 | Cysteine protease inhibitor,irriversible | Proteases|Cathepsin | 66701-25-5 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O |
| A2570 | Leupeptin, Microbial | Inhibitor of serine and cysteine proteases | Proteases|Serine Protease | 103476-89-7 | O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] |
| A2571 | Pepstatin A | Aspartic proteinases inhibitor | Proteases|Other Proteases | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O |
| B7854 | o-Phenanthroline | metalloprotease inhibitor | Proteases|Other Proteases | 66-71-7 | C(C=CC=N1)(C=C2)=C1C3=C2C=CC=N3 |
| Stored at -20°C, and stable for at least 12 months. | |||||
