In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
O-Phenanthroline is a membrane-permeable metal chelator, known as a non-specific matrix metalloproteinase (MMP) inhibitor .
Matrix metalloproteinases (MMPs) are zinc-dependent proteases involved in intra- and extra-cellular matrix remodeling resulting from an oxidative stress injury to the heart, of which MMP-2 increases during myocardial ischemia-reperfusion (I/R) injury-reduced oxidative stress, proteolyzing nuclear structural proteins.
In vitro proteolysis of lamins, which are the intermediate filament proteins providing support to the nuclear structural proteins and are targets of MMP-2, o-Phenanthroline abolished the proteolysis of lamin A .
The isolated rat hearts were subjected to ischemia-reperfusion (I/R) injury, of which treated with o-Phenanthroline had better left ventricular developed pressure and coronary flow during the reperfusion phase compared to those did not receive the drug .
 Georgi N, Landman E B M, Klein T J, et al. O-Phenanthroline as modulator of the hypoxic and catabolic response in cartilage tissue-engineering models [J]. J Tissue Eng Regen Med. 2017, 11(3): 724-732.
 Baghirova S, Hughes B G, Poirier M, et al. Nuclear matrix metalloproteinase-2 in the cardiomyocyte and the ischemic-reperfused heart [J]. J Mol Cell Cardiol. 2016, 94: 153-161.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||insoluble in H2O; ≥360.42 mg/mL in DMSO; ≥58.9 mg/mL in EtOH|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|
Quality Control & MSDS
- View current batch:
Purity = 99.90%
- COA (Certificate Of Analysis)
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)