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HyperScribe™ Poly (A) Tailing Kit

Catalog No.
K1053
used for polyadenylation of RNA transcription in vitro to enhance translation initiation efficiency
Grouped product items
SizePriceStock Qty
25 reactions
$160.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Product Citation

Quality Control

Quality Control & DataSheet

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Related Biological Data

HyperScribe™ Poly (A) Tailing Kit

Related Biological Data

HyperScribe™ Poly (A) Tailing Kit
 

Components and Storage

Amount Component Storage
100 uL E-PAP (2 units/uL) -20°C
600 uL 5X E-PAP Buffer -20°C
100 uL ATP Solution (100 mM) -20°C
250 uL 25 mM MnCl2 -20°C
10 uL Control DNA Template (0.5 ug/uL) -20°C
1 mL x 2 Nuclease - free Water any temp*

* Store Nuclease-free Water at -20°C, 4°C, or room temperature.

Description

HyperScribe™ Poly (A) Tailing Kit is a set of reagents designed to add a ≥150 base poly (A) tail to RNA transcripts generated with the HyperScribe™ T7 High Yield RNA Synthesis Kit. The kit is completed by using E. coli Poly (A) Polymerase (E-PAP) and ATP. Then the resulting capped and tailed RNA can be used in transfection or micro-injection experiments where mRNA stability and translation efficiency may be increased relative to unmodified mRNA.