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Z-VEID-FMK Caspase-6 inhibitor

Catalog No.A1923
Size Price Stock Qty
Evaluation Sample
$28.00
In stock
1mg
$110.00
In stock
5mg
$300.00
In stock
10mg
$500.00
In stock
25mg
$800.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Sample solution is provided at 25 µL, 10mM.

Product Citations

1. Xie J, Guo H, et al. "Identification of cleavage of NS5A of C-strain classical swine fever virus." Arch Virol. 2016 Oct 20. PMID:27766426
2. Chen Y, Sun M, et al, "a novel PAC-1 derivative, activates procaspase-3 and causes cancer cell apoptosis." Cancer Chemother Pharmacol. 2016 Aug 3. PMID:27488460

Quality Control

Chemical structure

Z-VEID-FMK

Related Biological Data

Z-VEID-FMK

Related Biological Data

Z-VEID-FMK

Biological Activity

Z-VEID-FMK is the specific recognition sequence for caspase-6/Mch2.
Targets caspase-6          
IC50            

Protocol

Cell experiment [1]:

Cell lines

Luteal cells

Preparation method

Soluble in DMSO. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reaction Conditions

50 μM; 6 h

Applications

Incubation of luteal cells in the presence of inhibitors of caspase-3 (Z-DEVD-FMK), caspase-6 (Z-VEID-FMK), caspase-8 (Z-IETD-FMK) and a general caspase inhibitor (Boc-DFMK) resulted in a reduction in the level of TNFα-induced DNA fragmentation.

References:

[1]. Abdo M1, Hisheh S, Dharmarajan A. Role of tumor necrosis factor-alpha and the modulating effect of the caspases in rat corpus luteum apoptosis. Biol Reprod. 2003 Apr;68(4):1241-8.

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Chemical Properties

Cas No. SDF Download SDF
Synonyms Caspase-6 inhibitor (fluoromethylketone),Benzyloxycarbonyl-Val-Glu(OMe)-Ile-​Asp(OMe)-fluoromethylketone
Chemical Name methyl (4S)-5-[[(2S)-1-[[(3S)-5-fluoro-1-methoxy-1,4-dioxopentan-3-yl]amino]-3-methyl-1-oxopentan-2-yl]amino]-4-[[(2S)-3-methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]-5-oxopentanoate
Canonical SMILES CCC(C)C(C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CCC(=O)OC)NC(=O)C(C(C)C)NC(=O)OCC1=CC=CC=C1
Formula C31H45FN4O10 M.Wt 652.71
Solubility Soluble in DMSO Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

Background

When compared to other caspase inhibitors, Z-DRHD-FMK inhibited caspase 6 activity more effectively than the general caspase inhibitor Z-Val-Ala-Asp (OMe)-fluoromethy ketone (Z-VAD-FMK) or the caspase 6 inhibitor Z-Val-Glu(Ome)-Ile-Asp(OMe)-fluoromethyl ketone (Z-VEID-FMK). However, it was less effective in inhibiting TNFα-induced apoptosis than Z-VAD-FMK or Z-VEID-FMK, presumably because caspase 6 is only one of at least three effector caspases, the others being caspase 3 and 7, that are active during caspase dependent apoptosis. Loss of DNA-binding activity and TNFα-induced apoptosis can be prevented by caspase-6-preferred inhibitor (Z-VEID-FMK)1.

The caspase-6-specific inhibitor Z-VEID- FMK and general caspase inhibitors significantly prevent apoptosis of caspase-6-microinjected neurons2.

Z-VEID-FMK, which inhibits caspase-6, was also able to abolish the cleavage of procaspase-8, although caspase-6 is activated downstream of caspase-8 in Fas-mediated apoptosis3.

References:
1. Nyormoi et al (2003) Sequence-based discovery of a synthetic peptide inhibitor of caspase 6. Apoptosis 8 371.
2. Y. Zhang C. Goodyer, Selective and Protracted Apoptosis in Human Primary Neurons Microinjected with Active Caspase-3, -6, -7, and -8. The Journal of Neuroscience, 2000, 20(22):8384–8389
3. Microinjected with Active Caspase-3, -6, -7, and -8 Taimen and Kallajoki (2003) NuMA and nuclear lamins behave differently in Fas-mediated apoptosis. J.Cell Sci.116 571.