Q-VD-OPh
Catalog No.
A1901
pan-caspase inhibitor
Featured Products
Q-VD-OPh (CAS 1135695-98-5) is a pan-caspase inhibitor targeting caspases including 1, 3, 8, and 9 with IC50 values ranging from approximately 25 to 400 nM. By blocking multiple caspase-mediated apoptotic pathways, including caspase-9/3, caspase-8/10, and caspase-12 pathways, Q-VD-OPh prevents apoptotic cell death induced by agents such as actinomycin D. In research contexts, this inhibitor serves as a tool to investigate apoptosis mechanisms across diverse cell types and species including human, mouse, and rat. It is also used to enhance cell viability upon thawing from cryopreservation under standard cryoprotectant conditions.
References:
1. T. M. Caserta, A. N. Smith, A. D. Gultice, M. A. Reedy and T. L. Brown, Q-VD-OPh, a broad spectrum caspase inhibitor with potent antiapoptotic properties, Apoptosis 2003; 8: 345–352
2. Yin XM. Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res 2000; 10: 161–167
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- 51. Joel Riley, Giovanni Quarato, et al. "Activated BAX/BAK enable mitochondrial inner membrane permeabilisation and mtDNA release during cell death."bioRxiv. 2018 February 26.
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| Storage | Store at -20°C |
| M.Wt | 513.49 |
| Cas No. | 1135695-98-5 |
| Formula | C26H25F2N3O6·xH2O |
| Solubility | ≥25.67 mg/mL in DMSO; insoluble in H2O; ≥28.75 mg/mL in EtOH |
| Chemical Name | (3S)-5-(2,6-difluorophenoxy)-3-[[(2S)-3-methyl-2-(quinoline-2-carbonylamino)butanoyl]amino]-4-oxopentanoic acid |
| SDF | Download SDF |
| Canonical SMILES | CC(C)C(C(=O)NC(CC(=O)O)C(=O)COC1=C(C=CC=C1F)F)NC(=O)C2=NC3=CC=CC=C3C=C2 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment[1]: | |
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Cell lines |
JURL-MK1 and HL60 cell |
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Preparation method |
The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months. |
|
Reaction Conditions |
No specific suggestion |
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Applications |
Q-VD-OPh largely inhibited caspase-3 and 7 activity at 0.05 mM. Caspase-8 was also inhibited by Q-VD-OPh at very low concentration. Q-VD-OPh prevented the cleavage of PARP-1 at 10 mM . Q-VD-OPh inhibited DNA fragmentation and disruption of the cell membrane functionality at 2 mM, and the drug-induced loss of cellular adhesivity to fibronectin need 10 mM Q-VD-OPh. |
| Animal experiment [2]: | |
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Animal models |
TgCRND8 mice in 3 months-old |
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Dosage form |
Intraperitoneally Injected with 10 mg/kg QVD-OPh at 3 times a week for 3 months |
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Applications |
Q-VD-OPh inhibited caspase-7 activation and limited the pathological changes of tau and caspase cleavage in chronic treatment of Alzheimer disease. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: 1. Kuželová K1, Grebeňová D, Brodská B.Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes. J Cell Biochem. 2011 Nov;112(11):3334-42. 2. Rohn TT, Kokoulina P, Eaton CR et al. Caspase activation in transgenic mice with Alzheimer-like pathology: results from a pilot study utilizing the caspase inhibitor, Q-VD-OPh. Int J Clin Exp Med. 2009 Nov 5;2(4):300-8. |
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| Q-VD-OPH is a selective, brain and cell permeable, highly potent and irreversible inhibitor of caspase-3 ( IC50=25nm), caspase-1 (IC50=50nM), caspase-8 (IC50=100nM) and caspase-9 (IC50=430nM). | ||||||
| Targets | caspase-1 | caspase-3 | caspase-8 | caspase-9 | ||
| IC50 | 50nM | 25nm | 100nM | 430nM | ||
Quality Control & MSDS
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